Bicinchoninic acid assay

Gravimetric Analysis of a Metal Carbonate Purpose The purpose of this lab is to find the mass of an unknown metal by conducting a double-displacement precipitation reaction, which is our gravimetric analysis. Once we find the precipitate mass, we can then find the molar mass by using the molar ratio given in the reaction equation. Procedure First, we set up the ring stand with a Bunsen burner, put a crucible on top of the pipe stem triangle and heated the crucible for a minute, then let it…

2.8.4 Properties The application of carrageenan as food additive depends on its performance such as forming thick solution or gels. For example, κ− carrageenan forms strong and rigid gels with potassium salts, forms brittle gels with calcium salts, clears gel with sugar addition, and exhibits syneresis. On the other hand, ι− carrageenan forms elastic gels with calcium salts, clears gel with no syneresis, and creates a gel that is freeze/thaw stable, while λ− carrageenan is not gel-forming but…

cells There are 3 main types of commercially available cells: 3.1.1Mono crystalline Silicon PV& Polycrystalline Silicon PV The occupational health issues are associated to chemical burns and inhalation of fumes from hydro fluoric acid (HF) and other solutions of acids (e.g., HNO3) and alkalis (e.g., NaOH) used for cleaning wafers ,removing complete oxide layer, and cleaning the reactor. Performing the slicing of wafers also results in solid waste for example (e.g., small pieces of…

Betacyanin pigments from beta vulgaris vacuoles were use in this experiment to determine the various effects of diffusion into its surrounding environment: 60%detergent, which is shown in the figures below. The four figures below are a set of data that were collected and analyzed the effects of time, salt concentration and ionic compositions on the rate of diffusion. Both figure one and two show a set of measurements between two different salt concentrations representing their absorbance versus…

In experiment 2, we observed that the reaction of halogenoalkanes with aqueous alkali and water which contains dissolved silver nitrate. Halogenoalkanes are alkanes which have one or more hydrogen atoms replaced by halogen atoms such as fluorine (F), chlorine (Cl), bromine (Br) and iodine (I) which are the elements in group 7 in periodic table. Halogenoalkanes have the general formula, RX, whereby R is an alkyl or substituted alkyl group and X is any of the halogen atom. Besides, halogenoalkanes…

solutions are remarkably resistant to pH changes and generally consist of a weak acid and its conjugate base or a weak base and its conjugate acid. In the laboratory, artificially made buffers are often used to help maintain a biological system at the proper pH. A laboratory buffer should be inert in the system being studied. For example, Tris buffer is unsuitable for some protein assays because it reacts with the assay components. Phosphate buffers contribute phosphate ions to a solution,…

dioxide. Nitrogen affects the growth of many proteins by all aquatic organisms. Oxygen is used by the organisms to breathe. Carbon dioxide is used by seaweed and other algae during photosynthesis. Also the levels of carbon dioxide affect the balance of acids and bases in saltwater when it produces carbonate ions under specific conditions (Duke University, n.d.). There is a lot of carbon dioxide in the ocean because of the burning of fossil fuels, coals, oils, and natural gases. The dumping of…

For much of human history, salt has been mined from the areas around dry lake beds and in deposits left after ancient seas dried up. These bodies of water all evaporated, leaving their crystallized salt deposits behind. Mined salt is sourced from these deposits. It differs from sea salt in that sea salt is taken from living oceans. While it is usually ground finely for sprinkling onto food as table salt, some of it is kept in a coarse and chunky form that is referred to as rock salt. Rock salt…

In this lab, we did four kind of different test, to mimic the chemistry of digestion. The first one was to test for sugar. We used a dropper and measured 2 ml of maltose and 2 ml of benedict’s solution in a pyrex test tube. After, using a test tube holder, we placed the test tube in a boiling water bath and heated for 5 min. After the 5 min, using a test tube holder we removed the test tube from the water bath. We then repeated the same procedure using starch solution instead of maltose. When we…

Practical 3: Enzyme Activity (2.5) Research Question: How does the change of pH affect the rate of decomposition of hydrogen peroxide on the enzyme, catalase? Background Information: Enzymes are globular proteins, which are synthesised by cells in order to perform specific functions. These act as catalysts in chemical reactions and are considered a major factor in an organism as these reaction rates are required to be shortened in order to support life. Catalase is one of the most…