16S ribosomal RNA

Overall Structure (Score 7) Dr. Glaunsinger divided her talk into four main sections over the course of her 50-minute talk. As her introduction, she appropriately began by providing a synopsis of her research by describing the mission of her lab and providing an overview of her interest in the interaction between DNA viruses and the eukaryotic genetic regulatory systems. To emphasize the broad goals of her lab, she introduced examples of model viruses, such as the gamma herpes viruses and MHV68…

Alkaline phosphatase (AP) is a homodimeric enzyme complex that is commonly found in a wide range of organisms, from bacteria to all tissues of the human body. AP is a zinc metalloenzyme (1), in which metal ions play a key role in the regulation of catalytic activities and stabilization of enzyme-substrate complex. As proposed by Gettins and Coleman using NMR studies (10), each active site of AP comprises of three metal binding sites, which acknowledged as M1, M2, and M3. Two zinc ions bind to…

a template for RNAs capable of recognizing viral DNA within a cell and as a means to bind the endonuclease CRISPR associated protein 9 (Cas9). These complexes allow the enzyme to be shuttled to the target DNA to perform a cut on one or both strands of the foreign DNA in either of the enzyme’s two active sites. Recently, scientists have repurposed this bacterial defense mechanism as a genome editing utility to cut, shuttle, or bind desired molecules anywhere within DNA as custom RNA harnesses the…

As stated previously, all that needs to be done is the guide RNA needs to lead the Cas9 enzyme to a particular location on a gene. An article in the New York Times stated that the system was so easy, that it could be mastered by a graduate student within an hour. This is a huge breakthrough when one takes into consideration…

Under oxidative conditions, certain nucleobases (for example, adenine and cytosine) can form N-oxides. Also, the C8 position of guanosine is vulnerable to hydrolytic attack under either strongly acidic or strongly alkaline conditions. The 5,6 double bond of pyrimidine nucleosides also reacts with halogens and halohydrins to give the corresponding addition products (Shabarova and Bogdanov, 1994). Selected examples of the side reactions that occur during oligonucleotide synthesis are given below.…

Leroy et al. (2000) used 4 microsatellite primers to characterise Brassica oleracea accessions. Among the 136 reproducible fragments generated, 25 (18.4%) fragments were common for all Brassica, 27 (19.9%) were unique and 84 (61.7%) were phylogenetically informative. Flannery et al. (2006) assessed polymorphisms in Brassica, Arabidopsis, Camelina, Raphanus and Sinapis using 10 plastid SSR primer sets. Eight loci were polymorphic, and separated the individuals of Brassicaceae into taxon-specific…

As aware of by most, one of the leading diseases that is causing a threat to our society currently is cancer. The prevalence of this disease is overwhelming and the treatment is possible; however, there is still need for further investigation into the cause of this disease before ¬¬¬¬¬it is thoroughly understood. Through increasing research there has been a connection made that cancer is the result of cells that continually replicate and causes for neoplasm to develop at an alarming rate (2).…

The cyano-ethoxy carbonyl protection Manoharan et al. (1999) developed 2(cyanoethoxycarbonyloxy)succinimide S.27a a stable, crystalline, and convenient reagent for the protection of pendant akylamines in oligonucleotides (Figure X.X.X). The 2-cyanoethyl (ce) group is the most common phosphate protecting group in oligonucleotides; however, ce and corresponding 2-(cyanoethoxycarbonyl) group were not utilized for the nucleobase protection until 2000. Merk et al. (2000) developed…

Protein structure prediction is that the prediction of the three-dimensional structure of a protein from its organic compound sequence — that's, the prediction of its folding and its secondary, tertiary, and quaternary structure from its primary structure. Structure prediction is essentially totally different from the inverse downside of protein style. protein structure prediction is one among the foremost necessary goals pursued by bioinformatics and theoretical chemistry; it's extremely…

6F4Z1003- Genetics, Adaptation and Diversity Practical Report Section 1- Calculations and Pedigrees 1. In DNA extraction the proteins absorb light at 260nm and 280nm, especially the aromatic acids. 2. 250µg to nm, 250 x 1000 = 250,000ng. 3. 200ng/ml = 0.2µg/ml. 150µg/ml = 150,000µg/ml 250,000pg/µl = 250µg/ml 1mg/ml = 1000µg/ml ● 150,00µg/ml - HIGHEST CONCENTRATION OF DNA ● 1000µg/ml ● 250µg/ml ● 0.2µg/ml - LOWEST CONCENTRATION OF DNA 4. Sodium dodecyl sulphate is used as a…