16S ribosomal RNA

To confirm the amplification of the 16S rRNA gene of the bacteria you use the performing gel electrophoresis. The PCR DNA is used to obtain a DNA sequence and use the DNA sequencing software to edit and use the NCBI database to find matching 16S rRNA gene sequence using BLASTn. There are many tests you can do to find our what your unknown bacteria are (Leicht, 2015). For this experiment my partner and I selected bacteria that was on our finger. We thumb printed the petri dish and let the bacteria grow on it. We did a visual classification of the DNA. We determined our bacteria were gram positive after doing the KOH string test. Then we preform additional testing using different growth mediums like vancomyin, EMB-lactose, PEA, and MSA, along with a catalase test. We also amplified the 16S rRNA gene from the unknown bacterial DNA used gel electrophoresis used Finch TV sequence to determine what the unknown bacteria is (Leicht, 2015). After preforming several tests that were expressed above, we ended up distinguishing that our unknown bacterium is Lactobacillus jensenii 269-3 (Altchul, 1990). Materials and Methods: My group and I obtain some bacteria from our fingerprints and different locations around the Biology Building. My partner and I examined our bacterial colonies that grew from the cells collected. We selected a single colony of the unknown bacteria from a fingerprint. We set up a PCR reaction to amplify the 16S ribosomal RNA gene, of the bacterial genome. To do…

A genotypic method uses molecular techniques to identify bacteria by doing DNA or RNA analysis of the bacterium’s genome ("Conventional Bacterial Identification Methods", 2013). The 16S rRNA gene sequences provides species-specific signature sequences useful for bacterial identification ("16S Ribosomal RNA Sequencing Theory”, 2016). The 16s rRNA gene sequence uses a polymerase chain reaction (PCR) to amplify a specific DNA target from a mixture of DNA molecules, which helps in the…

Some of the features that would be used for analysis were population, genotype, disease, nutrition, medication as well as environment and influence on disease. The key findings of the project would later be placed in a database that would be available to the scientific community. Hence sometimes the human microbiome project may be called a community project. One of the key methods used in the project was the targeting of 16s ribosomal RNA gene sequences these were used as taxonomic makers…

PCR is a common practice used to amplify a certain region of DNA. In this experiment, PCR was used to amplify the 16S ribosomal RNA gene of the bacteria. This locus was used because it is one that evolves slowly in bacteria. A Taq polymerase is used for this PCR because of the temperatures the reaction goes through. The16S rRNA primers used in this PCR, are complimentary to sequences conserved in all bacteria and will target sites of variation. These sites of variation were the regions of the…

can provide a more accurate picture of the diversity and structure of the whole microbial community. For bioturbation studies in particular, techniques such as denaturing gradient gel electrophoresis (DGGE), restriction fragment length polymorphism (RFLP), and ribosomal intergenic spacer analysis (RISA) have been used. Generally, diverse communities have been identified in burrow walls, and found to be more similar to either surface communities (Laverock et al., 2010, Pischedda et al., 2011) or…

transcriptional machinery of RNA Polymerase I in the oocyte Experimental Approach: Aim1: To address the roles of the Hira complex in rRNA transcription and embryonic development The Hira histone chaperone complex is an evolutionarily conserved complex, which is composed of Hira, Ubn1 and Cabin1, and cooperates with the histone chaperone Asf1a to mediate H3.3-specific binding and chromatin deposition12,13. I previously demonstrated the critical roles of maternal Hira /H3.3 on paternal…

In the present study, constitutive silencing of PMT genes was targeted to block or reduce the expression of putrescine methyl transferase that catalyzes the conversion of putrescine to methyl putrescine. As a consequence, accumulation of putrescine and nicotinic acid derivatives was expected. It was reported that silencing of PMT genes resulted in an increased level of putrescine and spermidine in Nicotiana sylvestris [14] and anatabine in Nicotiana tabacum [15]. Silencing of ADC, which converts…

for finding motif in genetic string. Introduction: DNA, RNA and Proteins, these are three types of very large molecules essential for every living organism, for their biological functioning. Each molecule, DNA, RNA and Protein, play a vital role in living organisms, without them no life could survive. Let’s understand functionality one by one: • DNA consists of encoded instructions which are necessary to maintain, assemble and reproduce. • Proteins play important role in movement,…

Photothermal therapy would support the nano-carriers with the RNA by creating a pathway through the cell to the nucleus. In which the nanomedicine will introduce foreign RNA to modify the genetics while the reactivity of the heat from the P.T therapy would enhance the strength of the nucleotide. There are two methods for this drug delivery. One takes place above 50 degrees Celsius and the other lingers around 45 degrees Celsius. Although the process and materials are the same, the results…

made up of smaller units called nucleotides. These nucleotides play a significant role in creating important structures found in our body such as deoxyribonucleic acid, more commonly referred to as DNA, and ribonucleic acid or RNA. (Erster, Lecture 4 Chapter 5) Ribonucleic acid is then categorized into two types of RNA: mRNA and tRNA (there are other types but the ones mainly discussed in protein synthesis only involved these two). The DNA and RNA are partners that work together to make protein…