Ubiquity, Aseptic Technique, And Pure Culture Isolation

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Ubiquity

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present everywhere

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Saprophytes

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Bacterial--> bacillus

Fungal --> mold yeast

Protozoan --> found in water

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Saprophytes

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microbes in nature which breakdown decaying organic materials, recycle nutrients.

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Commensals

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microbes that live on or in a organism that cause no harm with out any benefit.

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Mutualists

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Microbes that live on or in anorganism where both the microbe and thehost benefit.

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Obligate Pathogens

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Organisms that cause disease dueto their need to persist inside a host.

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Mutualists

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Microbes that live on or in anorganism where both the microbe and thehost benefit.

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Obligate pathogens

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organisms that cause disease due to their need to persist inside a host.

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Opportunistic Pathogens

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Microbes that can cause diseaseunder proper conditions. This group can includesaprophytes, commensals, and mutualists

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Culture

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group of microbes living together.

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culture meduim

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nutrient material for the growth of microbes.

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Media forms

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Solid--> use agar

liquid

semi-solid

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Nutrient Agar

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used to culture most bacteria

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Sabouraud's Dextrose Agar

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used to culture yeast and molds, prevents bacterial growth.

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Colony

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Billionsof cells that originate from one parent cell.

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Aseptic techniques

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Workarea disinfection.

Sterilizingloops or needles before and after inoculations.

Culturetube lip flaming before and after inoculations.

CoveringPetri plates during inoculation.

Workunder the flame of the Bunsen burner.

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Autoclave

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Pressure, Heat, Time!

Autoclaving uses heat and pressure. pressure allows rise in water temperature with out boiling.

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Pure culture

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when all cells or a colony or culture are descendant of a single organism.

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3 benefits of having a pure sample

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Macroscopic- colony morphology or cultural characteristics are species specific.

Microscopic- cell morphology can be easily identified and is unaffected by other organisms.

Physiological- test are true to the species being studied.

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3 things that isolated colonies can tell you

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isolate colony

use isolated colony for pure sample

determination if its pure

describe colony morphology