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16 Cards in this Set
- Front
- Back
the same gene of two individuals may be cleaved differently by a given restriction enzyme resulting in gene fragments of different legnth which is known as...
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restriction fragment length polymorphism (RFLP)
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what technique can be used to rapidly determine genotype of patient by hybridization of allele specific oligonucleotides to PCR amplified region of gene in question
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allele specific oligonucleotides
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hypervariable DNA contains a variable number of ____ which can be used to differentiate individuals
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tandem repeats
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what process can allow an individual to determine what genes are being expressed in a cell
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gene chips or microarrays
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gene delivery can occur via viral or non-viral vectors but non-viral has low ___ and viral vectors tend to induce ____
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efficiency/immune response
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what viral vector infects cells with high efficiency and integrates genes into genome, but only infects dividng cells and mest be rendered safe prior to use
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retrovirus
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what viral vector can infect dividing or non dividing cells, but is only transient and may induce immune response
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adenoviral vector
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which viral vector integrates gene into genome at defined site, is non-pathogenic, infects non-dividing cells, but lacks a good packaging cell line and only incorporates small DNA fragments
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adeno-associated virus
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what disease results in brain degeneration and death by about 10 years of age
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Canavan's disease
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what is needed to make a safe retrovirus
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helper provirus
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the helper provirus is unable to reproduce itself, but can express what viral proteins
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GAP, POL, and ENV
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is gene therapy more successful if there is no gene present or some gene present
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some gene present
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it was shown that an induced ___ caused the formation of leukemia in two SCID patients
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retrovirus
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10 patients have undergone Adenosine Deaminase Defeiciency gene therapy via retroviral vectors and what has been the affect
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increased immune function
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patients who were possible candidates for amputation were given ___ to stimulate angiogenesis
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VEGF
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in an effort to treat familieal hypercholesterolemia hepatocytes were treated with ___ that contained LDL receptor
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neurovirus
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