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56 Cards in this Set
- Front
- Back
Catalase (-) enzyme |
Does not have enzyme No bubbling EX: Entercoccus faecalis |
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Catalase + micrococci |
Should bubble Ex: Staphylococcus epidermis |
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Catalyst (-) |
Microbes _________ enzyme do NOT produce gas bubbles |
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Catalyst enzyme + |
Microbes with ________ produce oxygen gas bubbles when H2O2 is broken down |
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Fermentation |
Metabolic pathway which microbes produce ATP by breaking down glucose in absence of O2 |
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Acid ( pyruvic acid) Gas ( carbon dioxide) Alcohol |
Fermentation typically results in 3 things |
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pH indicator |
Production of acid can be detected by _________ |
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Phenol red ( PR) broth contains |
Peptone ( protein) Lactose ( carb) Duham tube pH indicator ( phenol red) |
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Deamination |
Process of bacterial break down of peptone Produces NH3 ( ammonia base) *removal of an amino acid |
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Lactose carbohydrates |
Bacteria are lactose fermenter Splits disaccharide lactose into glucose & galactose |
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Lactose |
Ferments glucose & galactose produces ATP , pyruvic acid & CO2 gas |
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Durham tube |
Tiny upside down tube collects CO2 gas bubbles produces during fermentation |
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Yellow & acid |
If pH is less than 7 : what color & type |
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Orange red Neutral |
If pH is equal to 7 What is Color & type |
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Base & pink |
If pH is greater than 7 |
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Catalase test |
Test identifies organism that produces catalase enzyme |
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Catalase |
Enzyme necessary to convert H2O2 to water & O2 |
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H2O2 |
Highly potent cellular toxin that forms during respiration pathways that invole oxygen |
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Aerobes Microaerophile Facultative anarobes |
Microbes that use O2 pathways are classified as: |
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Protective enzymes |
Catalase & superoxide dismutase are: |
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Catalase & superoxide dismutase |
Allow organism to live in presence of O2 |
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Differential test media |
Provide specific growth conditions where by organism carries out a reaction allowing it be distinguished from bacteria |
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Differential test media |
Used to differentiate Enterobacter species from each other and from other Gram (-) rods based on ability to ferment carbohydrates |
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Phenol Red Broth |
_____________ is a differential fermentation media |
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E Coli in Phenol red test
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Pink |
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Control in Phenol red test
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Red |
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S Flexnari in Phenol red test
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Gram + Yellow Acid No gas |
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K Pneumoniae
in Phenol red test |
yellow no gas |
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pink produces NH3 |
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Bacteria in Catalase test
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Enterococcus faecalis catalase - |
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Which bacteria had Good growth and which had bad growth in HE agar test?
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Color of growth for E Coli in HE Agar test
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Pi ( pink to orange) |
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Color of growth for E faecais in HE Agar test |
None, no growth present
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S flexnari color of gowth in HE Agar test
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Bppt ( Blue-green with black precipitate)
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color of growth for k pneumonia in HE Agar test
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Pi( pink to orange) |
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B ( blue green without black precipiate) |
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if you were trying to differentiate between Salmonella and Shigella which ingredient would need to be included in your media
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Substrate ( ferric ammonium citrate and sodium thiosulfate) |
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Is the control plate in HE Agar test lab, positive or negative and why?
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Positive control, set to show + results bacteria growth without inhibitor |
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When examining the results from all bacteria in HE Agar test, which bacteria is most helpful? |
E. Faecalis- because it has to show good culture |
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HE agar is both what type of media |
Selective and differential |
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selective media contain 4 components
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Inhibitor Substrate Indicator |
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selected to obtain optimum growth pf suspected organisms
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Nutrition |
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prevent or inhibit growth of some microbes |
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agent added that allows some bacteria to preform specific chemical reactions
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substrate |
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make desired reaction visable
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Indicator |
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HE agar contains what?
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Bile ( inhibitor) Ferric ammonium citrate & sodium thiosulfate ( substrate) Bromthymol Blue dye ( indicator) |
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Lactose ( nutrition ) |
Encourages growth of K pneumoniae and E coli |
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Bile ( Inhibitor)
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inhibits growth of Gram + |
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Ferric ammonium citrate & sodium thiosulfate ( substrate)
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form black precipiate in bacteria tht reduces sulfur |
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Salmonella |
sulfur reduction ( +) |
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Shigella
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sulfur reduction (-) |
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Bromthymol blue dye (Indicator )
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measures pH changes turns yellow in acid |
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E Coli is gram - or +
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lactose fermenter |
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E faeclais
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Gram + |
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S Flexnari |
Gram - sulfur reducer non lactose fermenter |
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K pnemoniae
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lactose fermenter |