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18 Cards in this Set
- Front
- Back
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Place these structures of the compound light microscope in the order that light passes through them on the way to the observer's eyes: (1) condenser, (2) ocular lens, (3) illuminator, (4) specimen, (5) objective lens.
A) illuminator → specimen → condenser → objective lens/ocular lens B) ocular lens → condenser → specimen → illuminator → objective lens C) illuminator → condenser → objective lens → specimen → ocular lens D) illuminator → condenser → specimen → objective lens/ocular lens |
D) illuminator → condenser → specimen → objective lens/ocular lens
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Related to focal length
A) Resolution B) Focus C) Contrast D) Immersion Oil |
B) Focus
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Label the microscope
A. stage B. clips C. tube D. condenser E. diaphragm F. objective lens G. eyepiece H. nose piece I. course focus knob J. fine focus knob K. arm L. base |
View image of microscope
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Which of the following statements about electron microscopy and light microscopy is FALSE?
A) The electron microscope has greater resolution than the light microscope. B) Both the electron microscope and the light microscope use the same wavelengths for illumination. C) Images produced by light microscopes can be in color, whereas images produced by electron microscopes are black and white unless they are artificially colored. D) Electron microscopes can allow examination of viruses and internal cell structures, whereas light microscopes are limited to objects that are 0.5 micrometers and larger. |
B) Both the electron microscope and the light microscope use the same wavelengths for illumination.
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What is the purpose of adding a drop of mineral oil to the slide?
A) To increase the magnification B) To lubricate the revolving nosepiece C) To reduce the amount of light rays that are refracted away from the objective lens D) To protect the lens from scratching |
C) To reduce the amount of light rays that are refracted away from the objective lens
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Which of the following is the most convenient and appropriate unit for expressing the size of an average bacterial cell?
A) centimeter B) micrometer C) nanometer D) millimeter |
B) micrometer
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Enhances the resolving power of the microscope.
A) Focus B) Resolution C) Contrast D) Immersion Oil |
D) Immersion Oil
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The total magnification achieved by the microscope is calculated by _____ the magnification of the objective and the ocular lens.
A) subtracting B) dividing C) adding D) multiplying |
D) multiplying
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A compound microscope uses two lenses at once to magnify the image of a specimen. The _____ lens is found in the eyepiece and the _____ lens is found in the revolving nosepiece.
A) oil immersion; ocular B) ocular; objective C) high-power; low-power D) scanning; oil immersion |
B) ocular; objective
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If you use a compound light microscope, a 2-micrometer bacterial cell is best seen at which magnification?
A) 10X B) 100X C) 400X D) 1000X |
D) 1000X
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When should you disinfect your workbench? Choose the best answer.
A) When all lab work has been completed. B) Any time you bring a liquid bacterial culture to your workbench. C) Before and after lab work. D) Only if a spill has occurred on your workbench. |
C) Before and after lab work.
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Which of the following actions should you AVOID in the laboratory?
A) Keeping a lab manual at your workspace. B) Placing culture tubes on their sides. C) Removing your gloves when you've completed the lab. D) Putting away unnecessary items. |
B) Placing culture tubes on their sides.
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Food and drinks are not allowed in the workspace primarily because they may __________.
A) distract you or others from lab work B) clutter your workbench C) spill D) transfer microbes into your body |
D) transfer microbes into your body
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What should you do if you need to leave the laboratory temporarily in the middle of your work?
A) Put all contaminated materials in the appropriate area for proper disposal. B) Decontaminate your work area. C) Put away your lab manual. D) Remove your gloves. |
D) Remove your gloves.
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Why should you decontaminate your work area prior to beginning lab work?
A) To prevent the potential transfer of microbes to you and your experiment. B) So that you don’t have to wear gloves during your experiments. C) To remove any microscope oil on your bench top prior to working. D) To coat the lab bench with disinfectant in case bacteria have spilled onto it. |
A) To prevent the potential transfer of microbes to you and your experiment.
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While working with a liquid bacterial culture, you get some culture on your gloves. Which of the following best describes what you should do?
A) Put on another pair of gloves over the contaminated pair. B) Clean your gloved hands with disinfectant. C) Remove your gloves, and properly dispose of them. Then put on a new pair. D) Wipe your gloved hands with a paper towel. Dispose of the paper towel in the autoclave bag. |
C) Remove your gloves, and properly dispose of them. Then put on a new pair.
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After using an inoculating loop or needle to transfer a culture from one medium to the next, what step should be taken next to avoid contamination?
A) Heat the inoculating loop or needle with the Bunsen burner flame until it becomes red hot B) Wipe the inoculating loop or needle with a paper towel C) Clean the inoculating loop or needle with soap and ethyl alcohol D) Carefully place the inoculating loop or needle onto bench top |
A) Heat the inoculating loop or needle with the Bunsen burner flame until it becomes red hot
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It is very important that microbiology students must develop aseptic techniques in order to prepare pure cultures. Which of the following statements properly defines aseptic technique?
A) A technique that involves antibiotic treatment B) A technique free of any microorganism C) A technique that involves treatment with ethyl alcohol D) A technique that is free from contaminating organisms |
D) A technique that is free from contaminating organisms
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