Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
68 Cards in this Set
- Front
- Back
The three basic type of hemolysis for Streptococcus species are:
|
alpha (incomplete) hemolysis - greenish color
beta (complete) hemolysis - transparent gamma (no) hemolysis - no effect |
|
Streptococcus Group A Streptococcus or Streptococcus pyogenes
hemolysis type:__________ catalase reaction:_________ gram POSITIVE cocci in:__________ TAXO-A:__________ PYR:__________ |
Streptococcus Group A Streptococcus or Streptococcus pyogenes
hemolysis type: beta catalase reaction: negative (-) [No bubble formation after adding H2O2] gram POSITIVE cocci in: chains TAXO-A: positive (+) PYR: positive (+) |
|
Streptococci pneumoniae
hemolysis type:__________ catalase reaction:__________ gram POSITIVE cocci in:__________ bile esculin:__________ Optochin disk or TAXO-P:__________ Bile solubility:__________ |
Streptococci pneumoniae
hemolysis type: alpha catalase reaction: negative (-) [No bubble formation after adding H2O2] gram POSITIVE cocci in: chains bile esculin: negative (-) [no black pigmentation] Optochin disk or TAXO-P: positive (+) [susceptible] Bile solubility: positive (+) |
|
Streptococcus Group B or Stretococcus agalactiae
hemolysis type:__________ catalase reaction:__________ gram POSITIVE cocci in:__________ CAMP TEST:__________ Hippurate hydrolysis:__________ |
Streptococcus Group B or Stretococcus agalactiae
hemolysis type: beta catalase reaction: negative (-) [No bubble formation after adding H2O2] gram POSITIVE cocci in: chains CAMP TEST: positive (+) Hippurate hydrolysis: positive (+) |
|
Streptococcus viridians
hemolysis type:__________ catalase reaction:__________ gram POSITIVE cocci in:__________ TAXO-P:__________ Bile solubility:__________ PYR:__________ |
Streptococcus viridians
hemolysis type: alpha catalase reaction: negative (-) [No bubble formation after adding H2O2] gram POSITIVE cocci in: chains TAXO-P: negative (-) [resistant] Bile solubility: negative (-) [insoluble] PYR: negative (-) |
|
Streptococcus Group D
hemolysis type:__________ catalase reaction:__________ gram POSITIVE cocci in:__________ TAXO-P:__________ Bile solubility:__________ PYR:__________ 6.5% NaCl:__________ Bile esculin:__________ |
Streptococcus Group D
hemolysis type: alpha/gamma catalase reaction: negative (-) [No bubble formation after adding H2O2] gram POSITIVE cocci in: chains TAXO-P: negative (-) Bile solubility: negative (-) PYR: negative (-) 6.5% NaCl: negative (-) Bile esculin: postive (+) [black pigmentation] |
|
Enterococcus spp./Group D streptococcus
hemolysis type:__________ catalase reaction:__________ gram POSITIVE cocci in:__________ TAXO-P:__________ Bile solubility:__________ PYR:__________ 6.5% NaCl:__________ Bile esculin:__________ |
Enterococcus spp./Group D streptococcus
hemolysis type: alpha/gamma catalase reaction: negative (-) [No bubble formation after adding H2O2] gram POSITIVE cocci in: chains TAXO-P: negative (-) Bile solubility: postive (+) PYR: postive (+) 6.5% NaCl: postive (+) Bile esculin: postive (+) [black pigmentation] |
|
What organism is the common cause of bacteria meningitis in neonates (newborns)?
|
Streptococcus Group B or agalactiae
|
|
What organism is the common cause of epidemic meningitis?
|
Neisseria meningtides
|
|
What organism is the common cause of bacteria meningitis in children?
|
Haemophilus influenzae
|
|
Haemophilus influenza
gram NEGATIVE:_________ oxidase:_________ catalase:_________ requires which factors:_________ grow only on which media:_________ |
Haemophilus influenza
gram NEGATIVE: coccobacilli oxidase: positive (+) catalase: positive (+) [bubble formation after adding H2O2] requires which factors: x and v factors grow only on which media: chocolate agar |
|
Neisseria gonorrhea
gram NEGATIVE:_________ oxidase:_________ catalase:_________ grow only on which media:_________ utitilizes which sugar:__________ |
Neisseria gonorrhea
gram NEGATIVE: diplococci oxidase: positive (+) catalase: positive (+) [bubble formation after adding H2O2] grow only on which media: chocolate agar, MTA (Modified Thayer Martin) utitilizes which sugar: glucose only |
|
What 2 organisms are used for satellism testing?
|
Haemophilus influenza
and Staphylococcus aureus |
|
Staphylococcus aureus
catalase test:__________ gram POSITIVE cocci in:__________ Slide Coagulase test:__________ Tube Coagulase test:__________ Mannitol salt agar:__________ |
Staphylococcus aureus
catalase test: positive (+) [bubble formation after adding H2O2] gram POSITIVE cocci in: clusters Slide Coagulase test: positive (+) [clumps] Tube Coagulase test: positive (+) [clumps] Mannitol salt agar: positive (+) [yellow colored colonies] |
|
Staphylococcus saprophyticus
catalase test:__________ gram POSITIVE cocci in:__________ Slide Coagulase test:__________ Tube Coagulase test:__________ Novobiocin results:__________ |
Staphylococcus saprophyticus
catalase test: positive (+) [bubble formation after adding H2O2] gram POSITIVE cocci in: clusters Slide Coagulase test: negative (-) [clumps] Tube Coagulase test: negative (-) [clumps] Novobiocin results: resistant |
|
Sheep blood agar plates or TSA-II (Tryptic Sol Agar) with 5% SB (Sheep's Blood) is used for which type of cultures:
a. __________ b. __________ c. __________ d. __________ e. __________ f. __________ g. __________ h. __________ |
a. throat cultures
b. sputum cultures c. urine cultures d. blood cultures e. CSF (cerebrospinal fluid)cultures f. wound cultures g. genital cultures h. ear/eye cultures |
|
What does a TSI (Triple Sugar Iron Agar) medium contain?
(From top to bottom) |
1) lactose, 2) surcose, 3) glucose, and Iron
|
|
Explain a A/A (acid on acid) TSI (Triple Sugar Iron Agar) medium.
|
Ferments lactose and/or sucrose and glucose turning the media all yellow
|
|
Explain a A/K (acid on alkaline) TSI (Triple Sugar Iron Agar) medium.
|
Ferments lactose and/or sucrose and does not ferment glucose, yellow slant and alkaline butt
|
|
Explain a K/A (alkaline on acid) TSI (Triple Sugar Iron Agar) medium.
|
Does not ferment lactose, may ferment sucrose and ferments glucose, alkaline slant and acid butt
|
|
Explain a K/K (alkaline on alkaline) TSI (Triple Sugar Iron Agar) medium.
|
does not ferment lactose, sucrose or glucose. Alkaline slant and alkaline butt
|
|
Explain a black colored TSI (Triple Sugar Iron Agar) medium.
|
produces H2S
|
|
MSA (Mannitol Salt Agar) is classified as which type of media?
|
selective and differntial
|
|
MSA (Mannitol Salt Agar) is used for the identification of which organism?
|
Staphylococcus aureus
|
|
Enrichment broth such as GN (gram-negative) broth or Selenite is used for cultures which __________ normal flora.
|
supresses
|
|
What test(s) can be used to identify Salmonella or Shigella species from other Enterobacteriaceae?
|
a. serological testing
b. bio-chemical testing |
|
What does IMViC stand for and which type of organisms are they use to identify?
|
I - Indole test
M - Methyl red test Vi - Voges-Proskauer C - Citrate Identifies: Enterobacteriaceae |
|
List the 10 genera of Enterobacteriaceae.
|
1) enterobacteriaceae coli (E. coli)
2) citrobacter 3) klebsiella 4) enterobacter 5) serratia 6) proteus 7) providencia 8) salmonella 9) shigella 10) yersinia |
|
What is the color apperance of Salmonella colonies on HE (Hektoen Enteric) plates?
|
black centered colonies witha green halo
|
|
What is the color apperance of Salmonella colonies on XLD (Xylose Lysine and Desoxychocolate) plates?
|
black centered colonies witha red halo
|
|
What is the color apperance of Salmonella colonies on MaConkey plates?
|
clear pink colonies
|
|
What is the color apperance of Shigella colonies on HE (Hektoen Enteric) plates?
|
clear green colonies
|
|
What is the color apperance of Shingella colonies on XLD (Xylose Lysine and Desoxychocolate) plates?
|
clear red colonies
|
|
What is the color apperance of Shingella colonies on MaConkey plates?
|
clear pink colonies
|
|
Salmonella spp., in addition to what they show on differnt plates, what will they will also produce?
|
H2S
|
|
Campylobacter jejuni isolated on a campy plate
catalase test:__________ oxidase reaction:__________ gram stain:__________ motility description:__________ |
Campylobacter jejuni isolated on a campy plate
catalase test: postive (+) oxidase reaction: positive (+) gram stain: gram negative rods, s shape, or seagull shape motility description: darting motility |
|
List 4 rapid methods used for the identification of Enterobacteriaceae.
|
1) enterotube II
2) API 3) Microscan 4) Vitek |
|
UA culture grows as follow: TSA (Tryptic Sol Agar) >100 colonies (same), MAC-NG (MaConkey agar plate - no growth), Enterococcal >100 colonies (same) PYR+ report as:
|
cc >or= 100,000 CFU(colony formed units)/mL Entercoccus species isolated,
ID (identify), and MIC (minimum inhibatory concentration) to follow |
|
UA culture grows as follow: >or= than three differnt colonies on the TSA (Tryptic Sol Agar), MAC-NG (MaConkey agar plate - no growth), Enterococcal-100 colonies report as:
|
greater than three organisms isolated, contaminated specimen. Please recollect.
|
|
UA culture grows as follow: TSA-NG, MAC-NG, Enterococcal-NG report as:
|
no growth at 24 hours
|
|
TSA > 100 colonies (same), MAC > 100 colonies (same), Enterococcal-NG report as:
|
cc >or= 100,000 CFU (colony formed units)/mL gram-negative bacilli isolated
ID (identify) and MIC (minimum inhibatory concentration) to follow |
|
List the three flagellates infections to humans?
|
1) Giardia lambia
2) Trichomonas vaginalis 3) Trypanosoma species |
|
How do humans get flagellates infections?
|
1) cysts contaminated in food or water. Fecal oral route.
2) STD 3) arthropod (insect) bite |
|
Amebic dysentery is caused by which significant pathogen?
|
Entamoeba histolyica
|
|
In which stage of an Entamoeba histolytica mutiplies in the intestine?
|
Trophozites
|
|
Which parasite is commonly identified by the anal impression-smear or cellophane tape method?
|
Enterobius vermicularis (pinworm)
|
|
A person becomes infected with tapeworms by eating the larvae infected in?
|
poorly cooked beef or pork
|
|
The permanent smear made for identifying parasites covered in lab is known as?
|
trichrome method (stain)
|
|
Chagas disease is caused by which parasite?
|
trypanosoma cruzi
|
|
List the characteristics and syndrome cause by Balantidium coli.
|
Ciliate protozoa, that causes dysentery-like syndrome
|
|
List the characteristics of tapeworms.
|
Ribbon like flatworms composted of individual segments (proglottids) each which contains both male and female sex organs. The scolex has hooklets and suckers.
|
|
List the basic structure of all protozoa.
|
The nucleus is well defined by a nuclear membrane, lying with cytoplasm, enclosed by a thin outer membrane
|
|
In malaria, the parasite has two life cycles, which one occurs in humans?
|
asexual in humans, sexual in mosquitoes
|
|
One group of round worms carried by arthtopods (insects) and enter the body by way of an insect bite is:
|
the filaria
|
|
What other organism is also gram-negative diplococci?
|
Moraxella catarrhalis
|
|
Syphilis is also know as?
|
Treponema pallidum
|
|
What organism produces mucoid colonies?
|
streptococcus pneumoniae
|
|
What organism produces an enzyme called pyrrolldonylaryl amidase?
|
Entercocci Group D species
|
|
What is the gram stain of Campylobacter spp.?
|
gram-negative curved rods
|
|
Campy plates are placed in what kinds of conditions?
|
Microaerophlic
|
|
Tapeworm in beef
|
taenia saginata
|
|
Tapeworm in pork
|
taenia solium
|
|
Coagulase Negative Staphlococcus (CNS)
catalase:__________ coagulase:__________ |
Coagulase Negative Staphlococcus (CNS)
catalase: postive (+) coagulase: negative (-) |
|
What organism(s) produce a swarming apperance on TSA + 5% SB?
|
p. mirabilis
p. vulgaris |
|
Urinary Tract infections in young women?
|
Staphlococcus saprophyticus
|
|
Staphlococcus spp. are __________ to TAXO-A (Bacitracin)
|
resistant
|
|
An adultworm is usually considered under what species?
|
Taenia spp.
|
|
Another name for tapeworms is...
|
Intestinal cestodes
|