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28 Cards in this Set
- Front
- Back
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BA |
Blood agar |
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SIM |
Sulfide indole motility medium |
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UB |
urea broth |
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Purpose of BA |
test for hemolytic activity |
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category of BA |
differential |
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Inoculation of BA |
quadrant streak |
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preparation of BA |
autoclaved, cooled, blood then added |
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reading for BA |
1. no growth 2. clear zone viewed from bottom=beta hemolysis 3. green/brown discoloration viewed from top =alpha hemolysis 4. no color change viewed from top=gamma hemolysis |
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purpose of SIM |
Screening for H2S, indole production, and motility |
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category of SIM |
biochemical |
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Inoculation for SIM |
stab |
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reagent used for SIM |
Kovac's reagent |
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reading criteria for SIM |
1.black precipitate= H2S (+) 2. add Kovac, if red =indole (+) 3. Cloudiness throughout the test tube=motility (+) |
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purpose of urea broth |
production of exoenzyme urease |
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category of UB |
biochemical |
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inolculation of UB |
dip and swirl |
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pH indicator of UB |
phenol red |
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reading criteria for UB |
Orange= urease (-) Fuschia = urease (+) |
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What is the EnteroPluri test? |
15 different biochemical tests for enterics |
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What are the 4 enterics? |
1. gram negative rods 2. facultative anaerobes 3. common intestinal bacteria which incl E.coli and related members of Enterobacteriaceae 4. glucose fermenting bacteria |
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Rapid screening test-Catalase |
Hydrogen peroxide: bubbling =catalase (+)-Differentiates Staphylococcus from Streptococcus |
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Rapid screening test -Coagulase |
Rabbit serum: Clumping=coagulase (+)-differentiates Staphylococcus aureus from other Staph species |
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gram positive coccus resident of digestive tract associated with UTI's can lead to endocartis abdominal infections at risk with long term hospitalization and catheders |
Enterococcus faecalis |
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gram negative rod coliform group produces vitamins K and B complex montezumas revenge |
Escherichia coli |
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gram negative rod lives in soil organic matter decomposer highly motile resistant to ampicillan and cephalosporin suseptible to trimethoprim |
Proteus vulgaris |
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gram positive cocci in grape like clusters assoc with skin and mucus membranes MRSA , VRSA |
Staphylococcus aureus |
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on the skin opportunist produce a slime layer which allow attachment to certain hospital apparatuses infections at site of prosthetic implantation |
Staphylococcus epidermidis |
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leading cause of UTI's -young women more suseptible to colonization hydrolyzes urea to produce amonia allowing growth in acidic environment spread through sexual intercourse |
Staphylococcus saprophyticus |
