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38 Cards in this Set
- Front
- Back
MAC |
MacConkey Agar |
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Purpose of MAC |
Isolation of gram negative enterics |
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Category of MAC |
Selective and differential |
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Inoculation of MAC |
quadrant streak |
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What is the special inoculation technique used only for the urine test? |
semiquantitative method using the volumetric loop |
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What are the special ingredients for MAC? |
SA=Bile salts and crystal violet DA=lactose pH indicator=neutral red |
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Reading criteria for MAC |
purple growth= (+)lactose fermentation colorless growth=(-) lactose fermentation |
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MSA |
Mannitol Salt Agar |
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Purpose of MSA |
isolates and differentiates halotolerant species |
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Category of MSA |
selective and differential |
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Inoculation technique for MSA |
quadrant streak |
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Special ingredients for MSA-mannitol salt agar |
SA=7.5 % NaCI DA= Mannitol pH indicator=phenol red |
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Reading criteria for MSA-mannitol salt agar |
1.growth=halotolerance, medium is lemon-yellow=mannitol fermentation (+) 2. growth=halotolerance, medium is pink =mannitol fermentation (-) 3. No growth=halotolerant (-) |
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reading of the urine streak |
min of 100k CFU determines an active UTI |
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OCD |
original cell density |
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Calculation of OCD |
CFU's divided by the loop volume ex: CFU/loop volume- 75 CFU/0.001 mL=OCD |
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Kirby Bauer Method |
bacterial lawn technique, a type of culture and sensitivity test |
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BA |
Blood Agar |
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Purpose of BA |
test for hemolytic activity |
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Category of BA |
Differential |
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Inoculation of BA |
quadrant streak method |
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Preparation of BA |
autoclaved, cooled, blood then added |
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SIM |
sulfide indole motility medium |
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purpose of SIM |
screening of H2S, indole production, and motility |
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category of SIM |
biochemical |
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incoluation of SIM |
stab |
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reagent of SIM |
Kovac's reagent |
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UB |
urea broth |
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purpose of UB |
production of exoenzyme urease |
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category of UB |
biochemical |
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inoculation of UB |
dip and swirl |
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pH indicator of UB |
Phenol red |
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gram positive coccus-resident of digestive tract-assoc with UTI-can lead to endocartis-abdominal infections-at risk with long term hospitalization and catheters. |
Enterococcus faecalis |
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gram negative rod, coliform group, produces vitamins k and B complex, montezumas revenge |
Escherichia coli |
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gram negative rod, lives in soil and water, organic matter decomposer, highly motile, opportunist of UG tract, nosocomial infections, resistant to ampicillan and cephalosporin, suseptible to trimethoprim |
Proteus vulgaris |
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gram positve cocci in grape like clusters, wide spread assoc with skin and mucus membranes, MRSA, VRSA |
Staphylococcus aureus |
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on the skin opportunist, strains produce a slime layer which allow attachment to certain hospital apparatuses, infections at site of prosthetic implantation |
Staphylococcus epidermidis |
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leading cause of UTI's-young women more susceptible to colonization-hydrolyzes urea to produce ammonia allowing growth in an acidic environment, spread through sexual intercourse |
Staphylococcus saprophyticus |