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22 Cards in this Set
- Front
- Back
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primary goals (lab diagnosis) |
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factors affecting the results |
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diagnosis is through (2) |
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10 specimens |
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(4) stool or fecal sample collection |
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8 factors to consider in the collection of stool materials |
1. intake of drugs (must be collected a week after intake; will leave crystalline residues that will interfere in examination) 2. antibiotics (affects number of protozoans) 3. amount of stool (formed - thumb size; watery - 5 to 6 tablespoon) 4. should be fresh (trophozoites may die in 30 mins) |
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affects number if protozoans |
antibiotics |
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amount of stool |
thumb size and watery (5-6 tablespoon) |
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may die is 30 mins |
trophozoites |
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may destroy protozoans |
contaminants (toilet water, urine) |
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may contain free-living parasites |
contaminants (soil and water) |
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refrigeration of stool |
3-5 C is acceptable (never freeze) arrest development of larvae prolong refrigeration causes desiccation |
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never keep stool inside incubator, true or false? |
true |
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preservation |
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stool to preservative ratio |
1 part to 3 parts preservative |
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8 common preservatives |
1. Formalin 2. Schaudinn's solution 3. Polyvinyl Alcohol (PVA) 4. Merthiolate-Iodine-Formalin 5. Sodium Acetate-acetic acid Formalin (SAF) 6. Formalin-Glycin 7. Lacto-phenol 8. Alcohol-Formol Acetic acid |
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Formalin |
5% protozoan cyst, 10% Helminths eggs |
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Schaudinn's solution |
used in stained preparation |
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Polyvinyl Alcohol (PVA) |
permanent stained prep. of protozoans |
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Merthiolate-Iodine-Formalin |
fixation of protozoans and helminths eggs |
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Stool/Fecal sample macroscopic/physical examination |
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stool/fecal microscopic examination |
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