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21 Cards in this Set

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what are the specimen of choice for smear preparation

EDTA anticoagulated whole blood or free-flowing capillary blood

What will you do if EDTa is used?

smears must be prepared within 1 hour of collection

Storing temperature of blood for smearing

18 C to 25 C

Types of Smear in Clinical Hematology Laboratory

Thin smear, thick smear and Buffy coat smear

Thin smear is also called

wedge smear, cover glass smear or spun smear

used to evaluate the type of anemia, to identify blood abnormalities, to screen presence of malarial parsite or to perform manual

Thin smear

to determine the species and the stage of the malarial parasite

Thick Smear

extremely low WBC count (<1.0x10^9/L)

Buffy coat smear

intracellular parasite that attacks RBC

Malarial oarasite

Wualities of a well made blood smear

6

Common causes of a poor blood smear

8

Romanowsky stain

3

Eosin

Components of Romanowsky stain

stain eosinophilic and basci structures

acidic component

eosin

stain basophilic structures and acidic structures

Methylene blue

Basuc component

Methylene blue

Cause of too Acidic smear

3

Cause of two basic smear

4

fix cells on the slide

Methanol

This is put after Methylene blue

Aged distilled water

prolinged dip into aged H20

Too acidic smear