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6 Cards in this Set
- Front
- Back
Outline the process of Gel Electrophoresis
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Extracting the DNA
Separating the DNA Visualising the DNA Isolating the gene of interest |
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Extracting the DNA
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DNA extracted
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Separating the DNA
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DNA cut by restriction enzymes placed in wells in polyacrylamide gel in buffer solution with electrodes at either end
DNA fragments travel along the gel to the ANODE or positively charged end according to size. DNA fragments thus separated |
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Visualising the DNA
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Polyacrylamide gel contains ethidium bromide which unites with the DNA and fluoresces under UV Light
DNA placed in dark room and UV light shone on DNA so can see fragments |
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Isolating the gene of interest
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A marker is loaded onto the gel
Details size of gene of interest Marker ocmpared to fragments to find gene of interest |
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Fate of the DNA after gel elecrtophoresis?
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The gene of interest could be removed from the gel, purified and then cloned
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