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121 Cards in this Set

  • Front
  • Back

TP/TP+FN= ?


TN/TN+FP=?


TP/TP+FP=?

SENSITIVITY


SPECIFICITY


PRECISION

What might the following indicate?urine: RBCs, WBCs, nitrite, bacteria

PYELONEPHRITIS

Why is albumin the first protein to be detected in tests for renal failure?

ITS MOLECULAR SIZE IS SMALLEST

Cortisol excess will result in _____

EXCESS GLUCOSE IN THE BLOOD

This spiral-form organism is seen in urine and cultured on Fletcher’s mediaa. Borreliab. Leptospira

Leptospira

Organism that gives off a bleach-like odor in culture?

EIKENELLA

Procedure #1 detected 50/100 true positives and 100/100 true negativesProcedure #2 detected 80/100 true positives and 70/100 true negatives

PROCEDURE 2 IS MORE SENSITIVE

What is the reason for the following discrepancy?Front TypePatient cells vs anti-A demonstrate a reaction strength of 3+Patient cells vs anti-B demonstrate a reaction strength of 3+Back TypePatient serum vs reagent A cells demonstrate a reaction strength of 3+Patient serum vs reagent B cells demonstrate a reaction strength of 0

THE PATIENT MAY BE A2B.

Presence of rheumatoid factor in blood may result in false positives for what test

VDRL.

Disease associated with the following results? Elevated TSH; Elevated T3; Elevated free T4

PITUITARY TUMOR

If excess PTH is released, what would you find in elevated amounts in serum

Calcium

Mucoid, pink colonies on plate; produces gas; indole (+). On TSI tube you see yellow on the slant and yellow in the deep. What organism is this?

Klebsiella Pneumoniae

PAD (+); indole (+); Organism stains gram negative. What is it?

P.vulgaris

You see a curved gram negative bacilli. It was cultured from the GI tract of a person with ulcers. What test would you do next to confirm its identity?

TEST FOR UREASE

Enzyme controls run on a machine give results around -3 standard deviations. Samples run on the same machine give results of less than 1 standard deviation. What could be the problem?

CONTROLS WERE LEFT AT ROOM T FOR SEVERAL DAYS

HIV-1 & HIV-2 combination ELISA test is positive in a patient with symptoms of immune deficiency. Western blot was inconclusive for HIV-1. What do you do next?

DO HIV 2 WESTERN BLOT

What are the steps of PCR?

denaturation, annealing, transcription

RAST test detects what?

IgE

After collecting a blood sample in an EDTA tube for CBC, you find that the Hematocrit is very high (67%). What should you do next?

REPORT THE RESULTS

When you conduct a procedure using fluorescence, it’s important to protect yourself from the

EXCITING LIGHT

Blood was collected on Nov 1. Blood was then frozen in glycerol on Nov 5. What should the expiration date read?

c. Nov 1; 10 years from now

22. A person was successfully treated for syphilis 12 years ago. However, he has just come in again, worried about having been re-infected. What would you look for in his blood?

VDRL

A patient demonstrates a positive antibody screen. You suspect either Jka, K or c antibodies. You know from a previous history that this patient has Jka antigen on their red cells. You then react the patients serum with cells positive for certain antigens and see the following:Patient serum vs: reagent K cells reagent c cellsReaction strength: 0 4+What can you conclude about the antigenic makeup of this patients red cells?

d. rule out c antigen but cannot confirm the presence or absence of K antigen on the patients red cells

Urine protein chemistry dipstick (Reagent strip) detected no proteins but sulfosalicylic acid (SSA) test did detect proteins. Why?

Bence-Jones proteins in urine

Syndrome of inappropriate antidiuretic hormone secretion (SIADH) would result in what in blood?

deficient sodium

Fiber strands in urine resemble what under the microscope?

Hyaline Cast

Which of the following regulates myocyte contraction?

TROPONIN

HBa1c levels cannot always be used to monitor glucose levels in conditions such as

SICKLE CELL DISEASE

What RBC inclusion can be seen on blood smear of a child who accidentally ingested moth balls?

HEINZ BODIES

Which of the following parasite cause autoinfection in immunocompromised px?

S.stercoralis

Which of the following causes antibody against TSH?

Hashimotos Thyroiditis

Which of the following Mycobacteria we can acquire from tap water?

M. Gordonae

Which of the following analytes is cofactor for most of 300 enzymes?

Zinc

Pigment found in the lungs of cystic fibrosis patients is called?

Pyocyanin/Pyoverdin

Specimen for Rotavirus?

Stool

Menstruation results in the decrease of what?

Ferritin

Most common cause of sperm agglutination is?

Presence of sperm antibodies

Which value is increased in mumps?

Amylase

What is a transudate and exudate?

Transudate= Extravascular fluid with low protein content. Exudate= Exudate is fluid that leaks around the cells of the capillaries caused by inflammation. High protein content.

Define Blastoconidia?

Budding of a yeast cell.

Malassezia Furfur?

OLIVE OIL

If a person undergoes salicylate overdose what would you test for?

pH DECREASE. METABOLIC ACIDOSIS.

ALP PH= 9.6 WHAT DISEASE IS PRESENT?

PAGETS.

Cocaine metabolite?

Benzoylecgonine

Which cells release heparin/histamine?

Basophils and mast cells.

Positive RPR negative FTA for syphillis

FALSE POSITIVE

Alpha Thalassemia is made up of?

Hgb Bart and Hgb H

Eosinophils in the Urine are seen in what condition?

Interstitial Nephritis

Isoagglutinin of Type O?

ANTI A B AND AB

HTLV confirmatory test is?

Western Blot

Latex agglutination for S. aureus factors seen?

Protein A and clumping factor

Potassium permanganate in auramine-rhodamine stain for Myco is what?

Quenching Agent

Iatrogenic anemia is caused by?

Multiple blood draws

RBC count very low and retic % is 0.1 what is this a hallmark of?

Pure red cell aplasia

HgbA1C values would be decreased in

Hemolytic Anemia

Lupus anticoagulant causes what?

Increased risk of thrombosis

Sample taken from indwelling catheter. Patient isn’t on any anticoagulants yet PTT and TT are way elevated. Cause?

Heparin

In which case is MG monitored?

Eclampsia

Patient taking primidone showing toxicity, but blood levels normal. What do you do next?

Test phenobarbital level.

Sperm count can be done on semen sample when?

When liquefaction is complete

Rouleaux is undetectable at what phase?

AHG

Sezary cells are associated with what cells?

T-cells.

Conns syndrome is characterized by an increase in?

Aldosterone

Virus transported for 92 hours or something = ?

LYOPHILIZED

Gram neg cocci present after jaw surgery

Veillonella

Micrcoccus is resistant to what?

Furazolidone

Procainamide metabolite is?

NAPA

Alpha thalassemia is made up of?

HGB Bart/Major

Blood product with highest capacity to transmit hepatitis?

Packed cells OR PLASMA

Heinz bodies are made up of?

Denatured Hemoglobin (DNA)

Beta Thalassemia Major is another name for?

Cooleys Anemia

Monosodium urate is highly?

Birefringent

Zygomycota? Geotrichum?

Sporangium. Arthroconidia.

Why is caffeine used in the diazo reaction?

To measure unconjucated bilirubin

How is measurement of iron carried out?

Step 1: addition of acid


step 2: addition of reducing agent


step 3: add color reagent

Fetal Lung maturity is assessed by examining what parameter?

Phosphatidylglycerol

Protein C what are you taking?

Warfarin

Case study about skin testing?

T-cells

Bilirubin reacts at what fluorescence?

450nm?

N.gonorrheae virulence factor?

Pili

A/A g+ what would you see on HE agar?

Orange colonies

Oral contraceptives are known for causing what?

Increase in serum FE.

What can cause turbidity in synovial fluid?

Crystals

A false positive urobilinogen is caused by?

Porphobilinogen

Caffeine benzoate in bilirubin assay is what?


Accelerator

Preferred specimen for Tacrolimus is?

Whole Blood

Preferred testing for legionella?

Urine antigen testing

Test for CMV?

Viral culture

Specimen for whooping cough?

Nasopharyngeal swab

Blood smear picture that looks like Howell bodies, the retic is 18%, the technologist should stain with?

Howell Jolly stain

Disease categorized by unconjugated bilirubin?

Gilberts Syndrome

Circulating anticoagulant?

Heparin

Fungal stain?

Cotton blue

Elevated levels of aminolevulinic acid in the urine is due to presence of?

Lead

What is added before doing cell count of synovial fluid?

Don't know :(.

What causes weak D?

Missing epitope.

Walking pneumonia has what effect on penicillin?

No effect

what nutrient is incorpriate in anaerobic ager to aid the growth of anaerobes

Glycerol and Iron

Difference between Yersinia pestis and entercolitica?

Motility

Why are first morning sputum needed to diagnose mycobacteria?

Because if the specimen is NOT first morning it will be invaded.

What instrument measures fluorescent light and transmits it at a large wavelength?

Fluorometer

Lewis antibodies in saliva of group A?

Le(a-b+)

5HIAA is what?


Serotonin metabolite. Seen in CARCINOID TUMORS.

Stain for cryptosporidium?

Trichrome

Give an example of a non fluorescing bacteria?

Pseudomonas

Clinitest result turns quickly from blue to orange and back to blue….is this test pos or neg

Negative

Electrophoresis SLE?

CENTROMERE

Using compensated polarized light, what is positive (blue) for birefringence?Uric Acid or Calcium pyrophosphate

Calcium Pyrophosphate

when a lab personnel is electrocuted. What to do when he/she is “stuck” to the electric source itself?

Disconnect the power source. Elevate feet and lower head.

Mother Le(a-b-), what would cause this? –

mother loses lewis antigens while pregnant

Bromide affects which electrolyte?

Potassium

Use of heparin manganese?

HDL precipitation

Patient with HA1C of 5%, glucose is 150.

Patient was following diet for beginning of 3 months and stopped.

Bacteria with tapered ends

Fusobacterium

Swarming bacteria, which test should you do next?

Indole for PROTEUS

11) A beta hemolytic gram positive cocci is growing on a blood plate. It is catalase positive, coag negative, oxidase negative, 6.5% NaCl positive.A) Report as normal floraB) Repeat the catalase and report out EnterococcusC) Repeat the coagulase and report out Staph aureusD) Repeat the oxidase and report out Micrococcus

B Repeat the catalase and report out Enterococcus

How long after whole blood donation should platelets be separated from RBCs?How long after whole blood donation should plasma be separated from RBCs?

5-8 hours

There was one question that asked what test hemolysis would effect the least!

Sodium

Indicator of nutrition?

Prealbumin

CSF should be stored at?

Room Temp

DOC SENDS A THROAT SWAB FOR ROTAVIRUS

CALL FOR CLARIFICATION (SHOULD BE STOOL)