Collection Tubes For Phlebotomy

Front 1

RED TOP

Back 1

ADDITIVE - None
MODE OF ACTION - Blood clots, & serum is separated by centrifugation
USES - Chemistries, Immunology & Serology, Blood Bank (crossmatch)

Front 2

GOLD TOP

Back 2

ADDITIVE - None
MODE OF ACTION - Serum separator tube (SST) contains a gel at the bottom to separate blood from serum on centrifugation
USES - Chemistries, Immunology & Serology

Front 3

LIGHT GREEN TOP

Back 3

ADDITIVE - Plasma Separating Tube (PST) w/ Lithium heparin
MODE OF ACTION - Anticoagulates w/ lithium heparin; Plasma is separated w/PST gel at the bottom of the tube.
USES - Chemistries

Front 4

PURPLE TOP

Back 4

ADDITIVE - EDTA
MODE OF ACTION - Forms calcium salts to remove calcium
USES - Hematology (CBC) & Blood Bank (Crossmatch); requires full draw / invert 8 times to prevent clotting & platelet clumping

Front 5

LIGHT BLUE TOP
(tube must be filled completely)

Back 5

ADDITIVE - Sodium citrate
MODE OF ACTION - Forms calcium salts to remove calcium
USES - Coagulation tests (protime & prothrombin time), full draw required.

Front 6

GREEN TOP

Back 6

Sodium heparin or lithium heparin, amonium heparin
MODE OF ACTION - Inactivates thrombin & thromboplastin
USES - For lithium level, use sodium heparin. For ammonia level, use sodiu or lithium heparin.

Front 7

DARK BLUE TOP

Back 7

ADDITIVE - EDTA
MODE OF ACTION - Tube is designed to contain no contaminating metals
USES - Trace element testing (zinc, copper, lead, mercury) & toxicology

Front 8

LIGHT GRAY TOP

Back 8

ADDITIVE - Sodium fluoride & potassium oxalate
MODE OF ACTION - Antiglycolytic agent preserves glucose up to 5 days.
USES - Glucoses, requires full draw (may cause hemolysis if short draw)

Front 9

YELLOW TOP

Back 9

ADDITIVE - ACD (acid-citrate-dextrose)
MODE OF ACTION - Complement inactivation
USES - HLA tissue typing, paternity testing, DNA studies

Front 10

YELLOW- BLACK TOP

Back 10

ADDITIVE - Broth mixture
MODE OF ACTION - Preserves viability of microorganisms
USES - Microbiology - aerobes, anaerobes, fungi

Front 11

BLACK TOP

Back 11

ADDITIVE - Sodium citrate (buffered)
MODE OF ACTION - Forms calcium salts to remove calcium
USES - Westergren Sedimentation Rate; requires full draw

Front 12

ORANGE TOP

Back 12

ADDITIVE - Thrombin
MODE OF ACTION - Quickly clots blood
USES - STAT serum chemistries

Front 13

LIGHT BROWN TOP

Back 13

ADDITIVE - Sodium heparin
MODE OF ACTION - Inactivates thrombin & thromboplastin; contains virtually no lead
USES - Serum lead determination

Front 14

PINK TOP

Back 14

ADDITIVE - Potassium EDTA
MODE OF ACTION - Forms calcium salts
USES - Immunohematology

Front 15

WHITE TOP

Back 15

ADDITIVE - Potassium EDTA
MODE OF ACTION - Forms calcium salts
USES - Molecular/ PCR & bDNA testing

Front 16

ORDER OF DRAW
FIRST TO LAST

Back 16

Light Blue (citrate)
Red/Black (gel, serum)
Red (no gel, serum)
Green or Tan (heparin)
Lavender or Tan (EDTA)
Royal Blue (EDTA)
Gray ( sodium flouride (glucose)
TUBES w/OTHER ADDITIVES
Yellow -drawn last (citrate ACD)

Front 17

MIXING CHART

Back 17

TUBE TYPE/ COLOR/ # INVER
EDTA / lavender / 8-10
Citrate / light blue/ 3-4
SSTw/gel / red-black/ 5
Serum / red / 5
Sodium Fluoride/ gray / 8-10
Heparin / green / 8-10

Front 18

SEPARATE
For serum (no get) tubes only

Back 18

CLOT- Lettube stand for clot to form at room temp for 60 sec.
SPIN - 10 - 15 minutes
POUR INTO TRANSPORT VIAL -label w/ specimen type