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16 Cards in this Set
- Front
- Back
Which way is the code read in proteins? |
5'-3' or N-C |
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What method was used to decipher the triplet code? |
In vitro translation assay |
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What are the 3 stop codons? |
UAA UAG UGA |
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How can you tell the genetic code is a non overlapping triplet code? |
3 mutations are needed to restore the reading frame |
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What advantage is there to a degenerate code? |
Creates a tolerance to mutations |
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What is the adapter in translation? |
tRNA |
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Where does the amino acid attach in a tRNA molecule? |
3' and 5' end |
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What is wobble base pairing? |
The way tRNA can recognise more than one codon because of variations in allowed patterns of hydrogen bonding |
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What is the purpose of the 2 ribosome subunits |
Peptide bind formation Codon matching |
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Name the 3 binding sites on a ribosome |
A - aminoacyl tRNA P - peptidyl- tRNA E - exit |
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Name the 4 steps in protein translation |
1. Complementary tRNA binds to mRNA codon 2. Peptide bond forms between previously bound amino acid and the current one 3. Large subunit translocation 4. Small subunit translocation |
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What catalyses the formation of peptide bonds |
Peptidyl transferase |
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What type of reaction occurs in the formation of peptide bonds? |
Condensation |
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How is translation ended? (3) |
1. Stop codon moves into A site 2. Release factor binds into A site 3. Water molecule added to the end of the amino acid chain |
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How long does it take to transcribe a 10kbp gene? |
10 mins |
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How long does it take to translate a 300 amino acid protein? |
1 min |