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18 Cards in this Set
- Front
- Back
Chromatin is a complex of _____ and protein. |
DNA |
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Genes in highly ordered (condensed) chromatin cannot be transcribed into _____and are relatively insensitive to DNase. |
RNA |
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The ________ is the basic subunit of chromatin. |
Nucleosome |
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Nucleosomes consist of a core particle, consisting of positively charged histone proteins, and of ______ bp of DNA wrapped twice around the outside of the core particle. |
150 bp |
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________ is condensed, inactive chromatin detectable by microscopy. Euchromatin is decondensed, active chromatin NOT detectable by microscopy.
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Heterochromatin |
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Histone acetyl transferases (HATs) add _______ to lysines, reducing the positive charge of the histone proteins and consequently reducing the interaction of the histone proteins with DNA. |
Acetyl Groups |
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Histone deacetyl transferases (HDACs) remove acetyl groups from ______ residues in histone molecules. Histone methylases add methyl groups to histones. |
Lysine |
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ATP-dependent remodeling complexes alter nucleosome structure(s) in a way that facilitates gene expression and requires _______. |
ATP |
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In the decondensed form, chromatin provides access to DNA binding proteins via the linker DNA, destabilized nucleosomes, or ________-free regions. |
Nucleosome |
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The ______________forms by an ordered assembly of components. Most of the components assemble by contacting other proteins of the PIC, not by contacting DNA. RNA pol II elongation proceeds by phosphorylation of the RNA pol II carboxy terminal domain (CTD) by TFIIH. |
Pre-Initiation Complex (PIC) |
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The Pre-Initiation Complex (PIC) forms by an ordered assembly of components. Most of the components assemble by contacting other proteins of the PIC, not by contacting DNA. RNA pol II elongation proceeds by __________ of the __________ by __________. |
Phosphorylation of the RNA Pol II Carboxy Terminal Domain (CTD) by TFIIH |
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The histone post-translational modifications can provide _________________ for ____________ that facilitate __________. For example, HP1 facilitates chromatin condensation and gene silencing. |
1) Binding Sites 2) Proteins 3) Chromatin Function |
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HATs, HDACs, histone methylases and ATP dependent chromatin remodeling complexes are brought to specific sequences in the DNA by SSDBP. We can call as SSDBP a “_________” when it leads to RNA transcription. |
Transactivator |
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_______, _______, _______, and _______ are brought to specific sequences in the DNA by SSDBP. We can call as SSDBP a Transactivator when it leads to RNA transcription. |
1) HATs 2) HDACs 3) Histone methylases 4) ATP Dependent Chromatin Remodeling Complexes |
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HATs, HDACs, histone methylases and ATP dependent chromatin remodeling complexes are brought to specific sequences in the DNA by ________. We can call as SSDBP a "Transactivator" when it leads to _________. |
1) SSDBP
2) RNA transcription |
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The __________ can provide binding sites for proteins that facilitate chromatin function. For example, _________ facilitates chromatin condensation and gene silencing. |
1) Histone Post-Translational Modifications 2) HP1 |
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___________ is a cartoon version of a chromosome that indicates chromosome map points that are based on chromosomal stains |
Ideogram |
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The___________ display always refers to a chromosomal ideogram at the top of the display. |
Genome Browser |