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30 Cards in this Set

  • Front
  • Back

Trypsin Tx.

Cyanogen Bromide Tx.

Chymotrypsin Tx.

Carboxypeptidase Tx.

Thermolysin Tx.

Pepsin Tx.

Endopeptidase Tx.

Elastase Tx.

2-Mercaptoethanol Tx.

4 Modes of Enzyme Regulation

4 types of Reversible Inhibition

Ki and Stability of Reversible Inhibitors

Enzyme Maturation

Ki and Stability of Irreversible Inhibitors

Allosteric Enzymes

Isoenzymes

Allosteric Effectors

Reversible Phosphorylation

Chymotrypsin Mechanism

Trypsin Tx. Of Chymotrypsinogen (target is Arg15)

Autolysis of (pi) Chymotrypsin

CM - Step 1

Substrate Binding

CM - Step 2

Nucleophilic Attack

CM - Step 3

Substrate Cleavage

CM - Step 4

Water Comes In

CM - Step 5

Water Attacks

CM - Step 6

Break-off from the Enzyme

CM - Step 7

Product Dissociates

Chymotrypsin - Catalytic Triad

His 57


Asp 102


Ser 195

Lysozyme - Enzymatic A.A.

Glu 35


Asp 52