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31 Cards in this Set
- Front
- Back
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proteome
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The total set of proteins encoded by a genome or the total protein complement of an organism
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translatome
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The total set of proteins that have actually been translated and are present in a cell under any particular set of conditions
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sodium dodecyl sulfate (SDS)
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Detergent used to unfold proteins and cover then with negative charges for electrophoresis
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Coomassie Blue
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A blue dye used to stain proteins
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isoelectric focusing
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Technique for separating proteins according to their charge by means of electrophoresis through a pH gradient.
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electrospray ionization (ESI)
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Type of mass spectrometry in which gas-phase ions are generated from ions in solution.
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Polyacrylamide gel electrophoresis (PAGE)
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Technique for separating proteins by electrophoresis on a gel made from polyacrylamide
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matrix-assisted laser desoption-ionization (MALDI)
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Type of mass spectrometry in which gas-phase ions are generated from a solid sample by a pulsed laser
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mass spectrometry
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Technique for measuring the mass of molecular ions derived from volatilized molecules
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time-of-flight (TOF)
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Type of mass spectrometry detector that measures the time for an ion to fly from the ion source to the detector
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Western Blot
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Detection method in which an antibody is used to identify a specific protein.
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avidin
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Protein from egg-white that binds biotin very tightly
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FLAG tag
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A short peptide tag (AspTyrLysAspAspAspAspLys) that is bound by a specific anti-FLAG antibody that may be attached to a resin for use in column purification or proteins
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glutathion-S-transferase
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Enzyme that binds to tripeptide, glutathione. GST is often used in making fusion proteins
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His tag
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Six tandem histidine residues that are fused to proteins so allowing purification by binding to nickel ions that are attached to a solid support. Also known as polyhistidine tag.
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maltose-binding protein (MBP)
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Protein of E. Coli that binds maltose during transport. MBP is often used in making fusion proteins.
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protein A
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Antibody binding protein from Staphylococcus that is often used in making fusion proteins.
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strepavidin
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Protein from Streptococcus that binds biotin very tightly
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tandem mass spectrometry (MS/MS)
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Two successive rounds of mass spectrometry in which a parent ion is first isolated and then fragmented in to daughter ions for more detailed analysis
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intein
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Self-splicing intervening sequence that is found in protein
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phage display
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fusion of a protein or peptide to the coat protein of a bacteriophage whose genome also carries the cloned gene encoding the protein. The protein is displayed on the outside of the virus particle and the corresponding gene is carried on the inside.
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biopanning
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Method of screening a phage display library for a desired displayed protein by binding to a bait molecule attached to a solid support
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phage display library
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collection of a large number of modified phages displaying different peptide or protein sequences
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protein interactome
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The total of all protein-protein interactions in a particular cell or organism
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two-hybrid system
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Method of screening for protein-protein interactions that uses fusion of the proteins being investigated to the two separate domains of a transcriptional activator protein
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bait
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The fusion between DNA binding domain of a transcriptional activator protein and another protein as used in two-hybrid screening
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prey
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The fusion between the activator domain of a transcriptional activator protein and another protein as used in two-hybrid screening
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co-immunoprecipitation
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Method of identifying protein-protein interaction by using antibodies to one of the proteins
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calmodulin
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A small calcium binding protein of animal cells
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metabolome
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The total complement of small molecules and metabolic intermediates of a cell or organism
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protein microarray
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Microarray of immobilized proteins used for proteome analysis and normally screened by fluorescent or radioactive labeling.
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