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19 Cards in this Set

  • Front
  • Back

Central Dogma of Biology

DNA is transcribed into RNA, RNA is translated into proteins

Polynucleotides

DNA and RNA; consist of phosphate backbone and different nitrogenous bases= nucleotides are linked by covalent bonds between phosphate of one nucleotide and sugar of the next

Nucleotide

Consist of a phosphate backbone, nitrogenous base, and sugar

Phosphodiester bonds

Link sugars and phosphates in nucleotide

Bonding order of nitrogenous bases

A to T (or U in RNA) and G to C

Nucleoside

Sugar and nitrogenous base

Purines

Adenine and Guanine

Pyrimidines

Cytosine, Thymine, Uracil

Difference between DNA and RNA in nucleotides

presence of hydroxyl group on 5c sugar in RNA

Order of synthesis of DNA strand

5' end to 3' end

DNA polymerase

Enzyme that adds activated nucleotides to template strand and also proofreads

Leading strand

3' end is far away from replication fork, is replicated continuously

Lagging strand

5' end is far away from replication fork; is replicated in fragments called Okazaki Fragments

Helicase

Enzyme complex that unwinds original DNA helix

Primase

Type of RNA polymerase which creates a RNA primer which in turn is used by DNA polymerase to replicate the DNA (usually done around 10 nucleotides at a time)

Ligase

Join segments of DNA/Okazaki fragments

Primer

Short segment of RNA synthesized by primes, eventually replaced by DNA

Origins of Replication

In order to replicate entire DNA, replication must begin at different places

Replication Fork

Site where DNA strands divide