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10 Cards in this Set
- Front
- Back
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Unknown Identification: -What was the process by which you isolated and identified your unknown sample? |
We obtained a TSB of our unknown bacteria, we do a smear on TSA, we incubate it and take it out the next day, we find and obtain a CFU to pick up aseptically. We do a gram stain and view it under the microscope as our first of many tests for the identification of our unknown. |
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Unknown Identification: -How would a flow chart be constructed for both Gram-positive and negative bacterial species? |
For Gram-Positive: Agar deep stabs, Litmus Milk Test, Starch Hydrolysis, (Urea Hydrolysis sometimes), Bacitracin (kinda)/Novobiocin Susceptibillity, Blood agar test, Coagulase test, Motility test. Catalase test. For Gram-Negative: Agar deep stabs, Litmus Milk Test, Indole Production, Methyl Red Test, Voges-Proskauer, Citrate Test, Urea Hydrolysis, Bacitracin/Novobiocin Susceptibillity, (Sometimes), SIM test. Catalase test. |
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What makes up the medium for a litmus milk test? -What us the Carbon/Protein source? -What is the Redox and pH indicator? |
Carbon source: Lactose Protein source: Casein Redox/Indicator: Azolitmin |
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-In a litmus milk test, when the medium is oxidized, what color will it turn? -What color will the medium turn when the medium is reduced? |
Oxidized medium: Blue or purple Reduced medium: white or colorless |
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-In the litmus milk test, when the pH changes, what color will the medium turn if the pH is below 4.5 (acidic)? -What color will the medium turn if the pH is between 4.5-8.3? -What color will the medium turn if the pH is above 8.3 (basic)? |
pH below 4.5: Pink pH between 4.5-8.3: Blue pH above 8.3: Purple |
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Can you spot the following reaction results? |
Oxidation/reduction of litmus,fermentation of lactose,gas formation, curd, acid clot, digestion, alkaline reaction, whey. |
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What are the controls for the litmus milk test? |
Lactococcus lactis: Pink (lactose ferm), acid clot (frim), white (litmus reduction) Bacillus subtilis: Soft clot turns to straw color whey with blue at top for alkalinization. Then clear. Escherichia coli: Pink (lactose ferm) pH 5-6 Alcaligenes faecalis: proteolysis, alkalinization Pseudomonas fluorescens: pH around 8, proteolysis, alkalinization. |
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What does Starch hydrolysis test for? |
To see if a microorganism can hydrolyses starch (duh-hoy) |
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What are the two forms of starch? |
Amylose (Linear) Amylopectin (Branched) |
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For the two forms of starch similar? (What subunits/linkages do they share?) |
Both forms of starch consists of: -alpha-D-glucose subunits -1,4-alpha-glycosidic bonds |
