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8 Cards in this Set
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- Back
Gel electrophoresis
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technique used to seperate individual components of a mixture of molecules using an electrical current
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In gel electrophoresis molecules must be ...
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water soluable and have at least a slight electrical charge
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agarose
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gelatin-like material
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buffer
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electrically conductive solution
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the buffer ____ of the molecules to be seperated and ____ the amount of ____ that passes through the chamber
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stabalizes the pH
controls electrical current |
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when an electrical current is passed through the chamber...
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the molecules in the mixture will begin to move towards the positive or negative poles of the chamber.
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Molecules with a negative charge will migrate towards _____ and those with a positive charge will migrate towards _____
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positive pole
negative pole |
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The agarose gel ____ of the molecules and acts like a ____
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slows the movement
sieve |