Bio 20A, Study Guide 10

Front 1

Restriction enzymes

Back 1

cut DNA at specific sequences -- very important for manipulating
DNA in the lab.

Front 2

Bacteria make restriction enzymes. The recognition sites for the cleavage are either 4 or 6
____ long.

Back 2

bases

Front 3

palindromic

Back 3

DNA sequence of both strands when read in the same orientation (5' to 3') is the same

Front 4

6 base recognition site

Back 4

4096 bases 6^4

Front 5

4 base recognition site

Back 5

256 bases 4^4

Front 6

_________ will not work on methylated DNA.

Back 6

Restriction enzymes

Front 7

When bacteria are infected by a ____, the _____ DNA enters the cell but is not
methylated. The restriction enzymes can chop up the _____ DNA and prevent it
from taking over the cell and turning it into a _____ factory.

Back 7

phage

Front 8

SNPs stands for:

Back 8

single nucleotide polymorphisms

Front 9

____ amplify
many regions with single nucleotide polymorphisms that affect restriction sites.

Back 9

PCR

Front 10

Agarose is derived from _____

Back 10

agar

Front 11

In an agrose gel, The _______charged DNA moves towards the ______ pole - _____ pieces move
_____ than longer ones.

Back 11

negatively ; positive; shorter ; faster

Front 12

RFLPs stand for

Back 12

Restriction Fragment Length Polymorphisms

Front 13

RFLPs can be used to identify:

Back 13

people and track genetic linkage and inheritance

Front 14

Plasmids

Back 14

small circles of double-stranded DNA - 3,000 to 10,000 bases long

Front 15

transformation

Back 15

the insertion of a plasmid into a bacterial cell

Front 16

overhangs

Back 16

can hydrogen bond together and the strands can be sealed with DNA ligase enzyme

Front 17

dideoxynucleotides are known as

Back 17

chain terminators.

Front 18

Reverse transcriptase

Back 18

makes a DNA copy of RNA