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Use of medium

Isolation and Enumeration of staphylococci spp

Media Composition


(Grams/L)

Tryptone-10 gm


Meat extract - gm


Yeast extract-1gm


Glycine - 12 ml


Lithium chloride-12 gm


Sodium pyruvate-10 gm


Potassium tellurite-0.21


egg yolk- 100ml


Agar -20 gm

Developed by

Baird Parker

Selective agent and it's action


Lithium Chloride and tellurite -Inhibit growth of other contaminants

Function of other ingredients

Tryptone,Meat extract - source of nitrogen, carbon, sulfur



Yeast extract -vitamin




Pyruvate and glycine - Enhances growth of injured staphylococci



pH of media

6.8-7.2

Incubation condition

35-37 °C for 24- 48 hrs

Cultural response

Coagulase positive Staphylococci produce black , shiny, convex colonies with entire margin and clear zone


If other contaminant grows they are grey or brown in color

Expected result

for more information


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