Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
11 Cards in this Set
- Front
- Back
non culturable strains |
strains that cannot be grown in a laboratory bc the appropriate growth conditions have not been discovered yet
|
|
most microbes on earth are |
non culturable |
|
16S rRNA |
RNA that is structural compoent of the small subunit of the ribosome |
|
Which sequences are more highly conserved? |
double stranded regions tend to be more highly conserved than sequences in the single stranded regions |
|
BLAST SEARCH |
t a database containing thed 16S rRNA gene sequences from all known species with THIS TOOL |
|
why is pCR needed |
to amply DNA to actually analyze |
|
BLAST steps |
1. genomic DNA isolated from clinical sample 2. PCR is used to amply 16S rRNA gene from this sample 3. DNA is sequenced 4. sequence is matched against the NCBI |
|
how to show 16SrRNA legit ness? |
bacteria DNA |
|
why is the 16S rRNA sequence commonly used for identifying bacteria? |
best thing to identify |
|
rDNA |
nucleoid |
|
rRNA |
ribosome subunit coded from rDNA |