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56 Cards in this Set
- Front
- Back
Just after decolorizer is applied acid-fast bacteria will appear ___ and non-acid bacteria will appear ______. |
Fushcia and colorless |
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After brilliant green is applied as counter stain, acid-fast bacteria will appear _____ while non-acid bacteria appear _____.
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Fuschia and green
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Mycobacterium is acid-fast ___ (positive/negative) and ____ shaped |
Positive bacillus |
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Appearance of Staphylococcus aureus after acid-fast stain |
Clusters of green cocci (acid-fast negative) |
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E. coli is gram ____ (negative/positive) |
positive |
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Most critical step of the gram stain |
Decolorization
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If you forgot to counter stain at the end of a gram-stain procedure, gram negative would appear ______ and gram positive would appear _______ |
Colorless and purple
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Microscopic morphology and Gram reaction for Staphylococcus aureus |
gram-positive staphylococci |
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Why is it important crystal violet be contrasting color to safranin |
Difference in color between the two stains allows you to differentiate between gram negative and gram positive cells by observing the color of organism |
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Microscopic morphology and Gram reaction for Micrococcus luteus |
gram-positive staphylococci |
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Purpose of allowing bacteria smear to dry when placed on slide |
Drying helps remove excess water to ensure optimal heat fixation and true arrangements are seen |
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Clusters of spherically shaped are called___ |
staphylococci |
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Chains of rod-shaped are called ____ |
Streptobacilli |
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Decolorizing agent in spore stain is _____ |
Water
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Name two acid-fast organism. |
Mycobacterium Nocardia |
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______ bacteria retain carbol fuchsin after acid-alcohol, ______ are decolorized |
Acid-fast non-acid fast |
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The bacilli in this stain are gram _____ |
negative (pink) |
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These cocci are gram _____ |
positive (purple) |
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These bacteria are acid-fast _____ |
positive (fuchsia) |
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Do these bacteria contain endospores? |
Yes, Endospore positive (pink with green) |
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These bacteria are acid-fast _____ |
negative (bluish-green) |
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Do these bacteria contain endospores? |
Endospore negative (pink) no green endospores |
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1. The genus of this organism is ____ 2. Describe the colonial morphology. |
Escherichia coli |
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1. The genus of this organism is ____ 2. Describe the colonial morphology. |
Pseudomonas |
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1. The genus of this organism is ____ 2. Describe the colonial morphology. |
1. Serratia |
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1. The genus of this organism is ____ 2. Describe the colonial morphology. |
1. Micrococcus |
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Fomite
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Object or substance capable of carrying infectious organisms |
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Aseptically |
Performed under sterile conditions
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Density
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Opaque, transparent, or translucent |
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Consistency
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Butyrous, mucoid, dull, dry, or powdery |
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Texture
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Smooth, rough, wrinkled, glistening, or irredescent
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Serial dilution
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Stepwise dilution of substance in solution |
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Colony forming unit- CFU
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Viable bacteria; colony |
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TFTC
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Too few to count
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TNTC
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Too numerous to count |
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Nosocomial infection
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Infection development favored by a hospital environment |
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Gram stain reagents, time, and purpose
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1. Crystal violet (primary stain)- 60 sec 2. Gram's iodine (mordant)-60 sec. 3. ethyl alcohol (decolorizer) 10-15 sec 4. safranin (counter stain) 30-60 sec |
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Acid-fast reagents, purpose, and time
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1. Carbol fuchsin (primary stain) 5 min 2. acid-alcohol (decolorizer) 30 sec 3. brilliant green (counter stain) 2 min |
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Endospore stain reagents and time
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1. Malachite green (endospore stain)-steam 5 min 2. safranin (vegetative stain)- 1 min |
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1. Name the organism we have seen in lab that could have been used to make this slide. 2. Describe the microscopic morphology
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1. Bacillus 2. Gram positive streptobacilli |
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1. The genus of this organism is ____ 2. Describe the colonial morphology. |
1. Mycobacterium 2. small, dry, pale yellow, rough |
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These bacteria are acid-fast _____ |
negative (blue-green) |
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1. The genus of this organism is ____ 2. Describe the colonial morphology. |
1. Bacillus |
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1. The genus of this organism is ____ 2. Describe the colonial morphology. |
1. Staphylococcus aureus 2. medium, opaque, golden, round, entire, convex, smooth |
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1. Name the 3 organisms we have seen in lab that could have been used to make this slide. 2. Describe the microscopic morphology
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1. Serratia, E. coli and Pseudomonas |
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1. Name the organisms we have seen in lab that could have been used to make this slide. 2. Describe the microscopic morphology |
1. Staphylococcus aureus Staphylococcus epidermidis 2. Gram positive staphylococci
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Escherichia coli- microscopic morphology _____ Endospore _____ |
bacillus gram negative acid-fast negative endospore negative |
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Staphylococcus aureus- microscopic morphology ______ Endospore _____ |
staphylococci gram positive acid-fast negative endospore negative |
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Micrococcus luteus- microscopic morphology _____ Endospore _____ |
cocci in tetrads gram positive acid-fast negative endospore negative |
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Bacillus subtilis- microscopic morphology ______ Endospore _____ |
streptobacilli gram positive acid-fast negative endospore positive |
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Serratia- microscopic morphology _____ Endospore _____ |
bacillus gram negative acid-fast negative endospore negative |
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Pseudomonas aerugenosa- microscopic morphology ______ Endospore _____ |
bacillus gram negative acid-fast negative endospore negative |
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Mycobacterium- microscopic morphology ______ Endospore _____ |
bacillus gram positive acid-fast positive endospore negative |
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1. Name the organism we have seen in lab that could have been used to make this slide. 2. Describe the microscopic morphology
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1. Micrococcus 2. Gram positive tetrads |
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Staphylococcus epidermidis- microscopic morphology ______ Gram _____ Acid-fast ____ Endospore _____ |
staphylococci gram positive acid-fast negative endospore negative |
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1. The genus and species of this organism is ____ 2. Describe the colonial morphology. |
1. Staphylococcus epidermidis 2. medium, round, opaque, flat, entire, white, creamy |