Polypeptide chains in the structural tertiary proteins are joined by Van Der wall forces and hydrogen bonds.
Polypeptide chains in the structural tertiary proteins are joined by Van Der wall forces and hydrogen bonds.
1.3 Identifying Knotted Proteins The complexity of protein folding makes it very challenging to fully understand the folding mechanism of proteins. More complex yet interesting challenges have been proposed. One of the most interesting challenges is the protein with knotted topology.(9-12) Knot is very common in our daily life. Sometimes we hate the knot and need to untie it.…
Conclusion The Albumin degradation experiment was used to see how fast a chemical reaction occurs when adding enzymes. In the results of the experiment we saw how the three tubes which had pepsin were faster than the one without pepsin. This differed from our hypothesis which stated the tube that only contained Pepsin would degrade the most. We also saw there was a difference between the sample that had sodium bicarbonate and acetic acid.…
Consequently, five test tubes were prepared at different temperatures to observe which test tube's temperature is the optimal temperature of the enzyme. These are the test tube and their temperature; Tube 1- was blank, Tube 2- room temperature, Tube 3- 35° C, Tube 4- 45° C and Tube 5- 55° C. These tubes had 1 mL of the substrate mixture and 1 mL of pH 6 buffer. These tubes were placed in a spectrophotometer to measure their reaction.…
Chemical Aspects of Digestion Lab Report By Abdulelah Almutairi Animal Form and Function Lab, 03, 12:30 PM Instructor: Melanie Gustafso-Ropski – Lab Assistant: Corinna Monday March 9,2015 1. Part A • The enzyme used in this experiment is pancreatic lipase. The substrate for pancreatic lipase is lipids or triglycerides.…
The effect of altering temperature on the rate of liver enzyme activity is measured in this experiment to be able to visualize how temperature in the environment and in the body can manipulate the rate of enzyme activity. Enzymes are protein catalysts that lower the amount of activation energy required to start a biochemical reaction. According to Dr. Meyertholen (2015), enzymes are structured to work with certain substrates, meaning that the structure determines the function of the enzyme. If the enzymes shape is altered, they become denatured. Denaturation of an enzyme can occur with changes in factors such as temperature, pH, or salinity.…
In most cases, a chemical reaction is able to occur in more than one way; enzyme kinetics studies the effect of how biological catalysts can influence biochemical reactions. Enzymes play an important role in regulating metabolic pathways in the human body and without enzymes they will not function efficiently. The rate of a reaction is enhanced by enzyme activity which provides alternate pathways for the reaction to proceed by.…
Discussion In this study, the Catechol enzyme was studied under the conditions of varying pH, temperature, substrate concentration, and enzyme concentration. In Figure 1, the data suggested that the trend was neither directly nor inversely proportional, but the highest activity rate was at 24°C. Most enzymes denatured at higher temperatures of approximately 40°C, which led to the inability to see any color change (Helms et al., 1998). At lower temperatures, the enzyme was somewhat efficient because molecules move slower at lower temperatures, so enzymes lost productivity.…
Introduction: Enzymes have a huge effect on the human body and other organisms. Enzymes are catalysts that are used to accelerate the reaction process by lowering the activation energy. For this experiment the catechol oxidase was extracted from potatoes. The enzyme is called catechol oxidase because the oxygen is reacting to catechol. Catechol oxidase causes the browning of the fruits and vegetables because the compound is toxic to the bacteria.…
Whitbread plc has many internal and external responsibilities it needs to meet. These responsibilities cover six main areas: • Key business responsibilities; • Legal responsibilities; • Social and ethical responsibilities; • Professional responsibilities; • Voluntary controls. Whitbread should ensure that, among other things, it: 1. Provides a safe working environment for its employees and adheres to Health & Safety legislation; 2. Deals fairly and honestly with its stakeholders; 3.…
Studying the reaction of the enzyme catalase in different temperatures The purpose of this experiment was to measure the speed and the effectiveness of the enzyme catalase. The enzyme catalase purpose is to break down harmful substances and turn them into oxygen and water. The substance in this environment was hydrogen peroxide, and the study was to see how well the chicken liver would work if it was in a boiled environment, room temperature environment, an acid immersed environment and a basic environment.…
The goal of this experiment was to determine the Michaelis constant (Km) and also the maximal velocity (Vmax) and the inhibition of alkaline phosphate. In order to accomplish these goals, 5 samples were used. Each sample contained different volumes of 0.2 m MPNPP (p-nitrophenylphosphate) and 0.2 M Tris-Hcl at a pH of 8.0. To each sample 0.2 mL of the enzyme studied (Alkaline Phosphatase) were added upon insertion on the spectrophotometer apparatus. With intervals of 20 seconds their absorbance at a wavelength of 410 nm was recorded at time frame of 2 minutes for each solution.…
Discussion: The specific purpose for this study was to see how enzyme activity would change as there was a change in temperature and pH. Both temperature and pH were hypothesized to have an affect on enzyme activity. Each had an optimal temperature. At this point, the rate of enzyme activity would stop increasing and begin to decrease. For temperature, it was hypothesized that the optimal temperature would be 48°C.…
Also, the requirement of enzyme activity on temperature has been labeled with two consisting processes: the catalytic reaction and the irreversible inactivation ( Biochem…
The purpose of conducting this experiment was to explore how different factors affect the reaction rate of enzymes reacting with their corresponding substrates in order to learn more about how enzymes function in different environments. The independent variables investigated in this experiment were the concentration of different substrates, the temperature of the environment, and the effect of a catalyst on the reaction rate. The dependent variable for all of the investigations was the time it took for the reaction to occur. To investigate the effect of the concentration of the substrate on the reaction time, four test tubes were used.…
Freezing and crystallization are linked in that both processes involve initial nucleation followed by crystallization. Sonication is thought to enhance both the nucleation rate and rate of crystal growth in a saturated or supercooled medium by producing a large number of nucleation sites in the medium throughout the ultrasonic exposure. Two main theories which explained the mechanism of nucleation are Hickling theory and molecular segregation theory. Among two theories Hickling is most commonly cited. According to this theory, violent collapses of cavitation bubbles produced by power ultrasound can generate very high localized pressure in a very short time.…