Examined T. californicus ability to handle high temperature stress under short-term, small exposure to high temperature, and long-term, persistant exposure to constant high temperatures. The took populations of T. californicus from 11 pools at different latitudinal gradients in a local scale. They put the specimen in petri dishes with founding adult females. They only mate one time and store the sperm so they can produce many groups of offspring. To test if inbreeding could influence the patterns of thermal tolerance they crossed independent lineages of the same population so they could use the offspring in thermal tolerance experiments. To determine the temperature of the stress trials they did experiments in the T. californicus natural
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They were put into 35 ◦ C to 39 ◦ C water for an hour. After that hour they were put in to 20 ◦ C water to bring the temperature back down. They were then transferred to a petri dish with food and put back in to 20 ◦ C water. They took note of how many survived. Experiments done before this one showed that the mortality rated from heat stress lowered. The generalized linear mixed models were compared with likelihood ratio tests to see if the added effects and interactions changed the models. For chronic temperature stress experiments they took 10 males and 10 females and put them in a petri dish and kept them at 32 ◦ C they added a little bit of flake fish food. They checked the petri dishes every day and recorded the amount of copepods that survived. Copepods from La Jolla and San Diego died within the second and third days. They tested competition in southern California and northern California populations. These experiments lasted several years at different temperature regiments. The experiments showed that females from Bodega Head, Santa Cruz, and San Diego survive and reproduce even in the highest temperatures. They also mixed the same number of females from two different populations into one petri dish so
They were put into 35 ◦ C to 39 ◦ C water for an hour. After that hour they were put in to 20 ◦ C water to bring the temperature back down. They were then transferred to a petri dish with food and put back in to 20 ◦ C water. They took note of how many survived. Experiments done before this one showed that the mortality rated from heat stress lowered. The generalized linear mixed models were compared with likelihood ratio tests to see if the added effects and interactions changed the models. For chronic temperature stress experiments they took 10 males and 10 females and put them in a petri dish and kept them at 32 ◦ C they added a little bit of flake fish food. They checked the petri dishes every day and recorded the amount of copepods that survived. Copepods from La Jolla and San Diego died within the second and third days. They tested competition in southern California and northern California populations. These experiments lasted several years at different temperature regiments. The experiments showed that females from Bodega Head, Santa Cruz, and San Diego survive and reproduce even in the highest temperatures. They also mixed the same number of females from two different populations into one petri dish so