its was higher at 8.5 3. State the optimum temperature for sucrase activity and describe how sucrase activity changes at lower and higher temperatures. Table 3: Effect of Sucrose Concentration on Sucrase Activity Optical Density 35 g/L 30 g/L 25 g/L 20 g/L 15 g/L 10 g/L 5 g/L 0 g/L 1 0.984 1.011 0.948 0.894 0.821 0.773 0.500 0.002 2 0.985 0.995 0.945 0.931 0.821 0.772 0.501 0.003 3 0.981 1.005 0.945 0.924 0.826 0.736 0.524 0.001 average 0.983 1.000 0.946 0.916 0.823 0.760 0.508 0.002 Laboratory Report/ Angela Collins/ Enzyme Activity/ Catherine Rice/ 07.09.2014/ Page [2] of…
The Pressure in the bag reduces due to a loss of volume of the solvent. A point reaches whereby the pressure on the outwards side of the tubing equal to the force driving the water out of the tube. At this point, no more osmosis occurs in the bag (Wisniak, 2013). Bag B stimulates a cell whose contents are isotonic to its surrounding environment. This situation arises because the concentration of the bag contents is 1% sucrose concentration that resembles the solute concentration on the outside.…
While he wanted to achieve a solution of 400 mL, he ended up with a solution of 420 mL because he did not take into account the volume that the solute (sucrose) would take up within the solution. This additional volume would thus also change the concentration; however, even if the actual concentration amounted to 400 mL, he would still have not obtained the 4M he assumed he was creating. This may be due to guessing, but he may have also assumed that by adding 400 mL of water, that would make the solution 4 M, regardless of the amount of solute he added into the mixture (If he wanted a 3M solution, he may have thought he could add 300 mL of deionized water). Because of his error, he ended up with a solution with a much smaller concentration. Therefore, when the student wants to create a solution of certain concentration, he needs to know either how much solution or solute he wants and then calculate for the other component in order to achieve the desired concentration.…
My results do not really make sense due to the fact that they didn't go for the glucose solution maybe it wasn’t a high enough concentration to attract…
However, when the substrate concentration was increased, the intensity of color stopped increasing due to the enzymes being unable to to process at a faster rate. All of the alternate hypothesizes were supported; in the experiments, section one (with Figure 1) had an optimal temperature at approximately 24°C; section two (with Figure 2) had an optimal pH at approximately pH8; in section 3 (with Figure 3) the color continued to increase with the addition of more enzymes; and in section 4 (with Figure 4) the color continued to increase until it reached a maximum velocity with the addition of more substrates. One desired retrial for the experiment would be to test for more points after 20 drops of potato juice in Figure 3. Understanding the effects of how these properties alter the ability of the catecholase enzyme could allow for better insight into decreasing time needed for biochemical reactions, proper food storage, and the condition of…
The result of this experiment was that tube 5 and 6 decreased in mass. Tube 2, 3 and 4 increased in mass. This is because in the hypertonic solution, water in the tube was flowing outwards, because the concentration of dissolved solutes was greater outside the tube than inside it. This caused the tube to shrink, for example, beaker number 5 and 6 with 40 % sucrose outside and 20% inside the tube. Apart from this, in hypotonic solution, water was rushed into the tube by osmosis, which is not good for the cell because it will fill with water and burst, for…
Part B Two test tubes were labeled, one with “yeast” and the other with “water”. 1 ml of yeast suspension and 4 ml of distilled water was added to the “yeast’ labeled tube. 5 ml of distilled water was added to the “water” tube. 5 ml of sucrose solution was added to each test tube and stirred.…
Observing Osmosis INTRODUCTION Osmosis is the “diffusion of water across a selectively permeable membrane” (Raven et al. G-16). In this experiment, dialysis tubing was used to mimic the selectively permeable membrane to show what osmosis does when placed in different solutes. We filled dialysis tubes fill with different percentages of solute and placed them in beakers full of a different solute. Through this experiment, I saw what happens when the solution becomes hypertonic, hypotonic, and isotonic. My hypothesis was that bag A would shrink because the water would leave the bag to go to the higher concentration.…
The purpose of this EEI is to create and analyse an experiment that relates to and supports the theory of osmosis in biological cells. Cells are the biological building block for all life; all living organisms have cells also. Cells provide structure, absorb nutrients from food and convert those nutrients into energy so that the cell can carry out specialized functions. There are two main categories with most organisms and their cells; prokaryotes and eukaryotes. The distinctive difference between prokaryotes and eukaryotes is that eukaryotic cells contain membrane-bound organelles, such as the nucleus, while as prokaryotic cells do not.…
The purpose of this experiment is to demonstrate the molar mass by using the freezing points of the two solutions. Colligative properties were used in calculations during this experiment to discover the molar mass of an unknown solute. These properties are subsequently applied to solutions depending on the amount of particles of the solute and the solvent inside the solution. In this lab, we focus on the freezing point depression, which can be seen when a pure solvent is added to or mixed with a solute, which will then drop the temperature. Therefore, an increase of liquefied solute would lower the freezing point.…
Twenty equally sized chickpeas were weighed out and placed into a 250 mL beaker. Once the chickpeas were weighed out, 100 mL of 0 M, 1 M, and 3 M concentrated NaCl solutions were made. To ensure adequate hydrolysis, the chickpeas were covered with at least two to three centimeters of concentrated NaCl solution. A total of two trials of each concentrated NaCl solution were conducted over a total time period of 160 minutes. Besides the initial temperature of the solution, data was collected at 5 minutes and time intervals of ten minutes until 60 minutes have been reached, increasing the intervals to every twenty minutes until 160 minutes approaches.…
This occurs because according to osmosis, water moves down its concentration gradient from a low solute concentration to a solution with a high solute concentration. In addition, an increase in the mass of the potatoes occurs because the highly concentrated solutions were hypertonic. Potatoes with the greater increase tend to be the sweet potatoes because the potatoes already have sucrose embedded in the substance. When placed in a solution with high sucrose content, the amount of sucrose in the sweet potatoes increases because the solutes enters a cell with higher solute potential than that of the surrounding environment. When the net osmosis rate is zero the solution is isotonic because equal amounts of solute is present in the cell and the surrounding environment causing no net movement.…
The purpose of conducting this experiment was to explore how different factors affect the reaction rate of enzymes reacting with their corresponding substrates in order to learn more about how enzymes function in different environments. The independent variables investigated in this experiment were the concentration of different substrates, the temperature of the environment, and the effect of a catalyst on the reaction rate. The dependent variable for all of the investigations was the time it took for the reaction to occur. To investigate the effect of the concentration of the substrate on the reaction time, four test tubes were used.…
More than 65% of ovules were just enlarged without undergoing callus or shoot phase independent of the sucrose concentration. Rest of the ovules was either with no development or dead after 8 weeks of culture period. The effect of sucrose concentration on ovule response was not pronounced (Table 18). When comparing the suitability of sucrose concentrations (2% or 4%, 4% 0r 6%, 2% or 6%) for ovule culture depending on the genotype, there is no significant difference for both genotypes on whatever the sucrose concentrations used (Table 19). No callus or shoots were obtained in this experiment.…
INTRODUCTION Cellular respiration is the breaking down of organic molecules by means of catabolic pathways that uses electron transport chain to produce ATP (Campbell 2011). ATP is used as energy in the synthesis of light or also known as Photosynthesis . ATP is used by photosynthetic organisms to produce their own food and also by performing cellular respiration. It needs mainly glucose and oxygen that involves the transfer of electrons in a chemical reaction.…