Replica Plating Lab Report

It has been proven that yeast can grow on both YPD and SD plates. In this lab we cross several yeast strains through the process called replica plating. For this identification test only one dominant allele is required. One copy of each gene is essential to produce enough of each enzyme needed in order to make a histidine. Through the study of Saccharomyces cerevisiae we prove that by transferring yeast cells onto YPD and SD plates over a period of 24-72 hours in a thirty degrees Celsius incubator, in return will grow yeast.

Introduction

Yeast can reproduce both asexually and sexually, which makes it very easy to grow in the laboratory, as it is very small in size. Mutant yeast can be easily isolated considering yeast consists of a single cell and can be grown as a haploid or diploid. Diploid cells are formed by the combination of MATa and MAT alpha cells. However, under …show more content…
Replica plating consists of using sterile velvet placed onto the replica block with the ring holding the velvet in place. Gently place the media and colonies down onto the velvet leaving a copy of the yeast on the velvet in order to transfer to another plate. Only use the velvet once per plate to avoid contamination. You can place either an YPD, SD, SD+His, SD+His+Leu, SD+His+Ura, and SD+His+Leu+Ura plates. An YPD plate contains all amino acids while an SD plate does not contain amino acids and only contains the essential nutrients. The SD+ plates will contain either an amino acid such as histidine or leucine, or a nitrogenous base such as uracil or any combination of the three. In order for yeast to grow on any of these plates, they need to be placed in an incubator at thirty degrees Celsius. If you wish to slow down or stall the growth of the yeast you may place the plates at four degrees Celsius, which is a temperature that can be obtained in a