Title: Exploring the Factors Affecting Post-Wound Hair Regeneration in Mice.
Hypothesis: We hypothesize that in mice large wound size increases the number of hair follicle because large wound will activate the Wnt pathway that is necessary for hair follicle regeneration in wounds.
Independent variables being tested: The wound size of the mice.
Dependent variables being tested: The number of hair follicle.
Experimental Design:
This experiment will be designed to determine if large wound size, would affect the number of hair follicle. This experiment will have 60 genetically normal Mus musculus and 30 transgenic mice that over express Wnt signaling pathway. A total of 90 black mice from both sexes ranging in age from 50 to 150 days will be purchased from the Jackson Laboratory. Animals are going to be either genetically normal or transgenic mice. All animals will be fed a normal chow diet and are going to be group-housed at a vaivarium. …show more content…
The first group will contain 30 genetically normal mice. Following the hair removal on the dorsal side of the mice, a 1cm in diameter wound size made using a punch biopsy (Pan et al., 2018). The first group will be used to compare our results to the experimental group because according to a study by Ito a 1cm in diameter wound will fail to regenerate hair follicle (Ito et al., 2007). To test the effect of large wound size on the hair follicle regeneration, second group will include 30 genetically normal mice. A hair removal will be done followed by a 3cm in diameter wound size using a punch biopsy. Lastly, to confirm the experimental results, third group will contain transgenic mice that over express Wnt
Hypothesis: We hypothesize that in mice large wound size increases the number of hair follicle because large wound will activate the Wnt pathway that is necessary for hair follicle regeneration in wounds.
Independent variables being tested: The wound size of the mice.
Dependent variables being tested: The number of hair follicle.
Experimental Design:
This experiment will be designed to determine if large wound size, would affect the number of hair follicle. This experiment will have 60 genetically normal Mus musculus and 30 transgenic mice that over express Wnt signaling pathway. A total of 90 black mice from both sexes ranging in age from 50 to 150 days will be purchased from the Jackson Laboratory. Animals are going to be either genetically normal or transgenic mice. All animals will be fed a normal chow diet and are going to be group-housed at a vaivarium. …show more content…
The first group will contain 30 genetically normal mice. Following the hair removal on the dorsal side of the mice, a 1cm in diameter wound size made using a punch biopsy (Pan et al., 2018). The first group will be used to compare our results to the experimental group because according to a study by Ito a 1cm in diameter wound will fail to regenerate hair follicle (Ito et al., 2007). To test the effect of large wound size on the hair follicle regeneration, second group will include 30 genetically normal mice. A hair removal will be done followed by a 3cm in diameter wound size using a punch biopsy. Lastly, to confirm the experimental results, third group will contain transgenic mice that over express Wnt