Ninhydrin Research Paper

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1-Paper
2-gallon size Zip- Loc bag
3-Iodine beads (available from forensic supply companies) Note: Iodine crystals can be used along with a small amount of powdered vermiculite.
4-Rubber or plastic gloves are recommended
5-Safety glasses or goggles
Safety Precautions
Firstly,Wear safety glasses or goggles to protect our eyes from iodine vapors. Iodine is very toxic in nature , both as vapor and through excessive skin contact.
Work in a well ventilated area is necessary. We must Keep all containers closed. A small amount of iodine on the skin can be removed with soapy water, but it will leave an orange colored stain. Dispose of any iodine waste in a safe manner.
Procedure
Have someone write a note on a sheet of paper. Generally, they leave
…show more content…
Ninhydrin is very irritant. Ninhydrin will react with any protein, particularly with that on your skin. Wear disposable plastic gloves and work in a fume hood. If we are sloppy, you will have purple hands or skin for over a week.
Use the ninhydrin solution in low light conditions only.
Procedure
Add water to the steam iron. Turn on the steam iron and set it for cotton. Have someone handle or write a note on a sheet of paper. Generally, they leave fingerprints on the paper as they hold the paper in place with one hand while writing with the other hand.

Handle the paper from the edges only, preferably using tongs, or wear rubber gloves. Place the paper on a sheet of newspaper or other surface covering the bench top under a hood.
Spray the paper lightly with the ninhydrin solution. After this,allow the paper to dry. Place the dry paper on a cloth towel. Cover with a second cloth towel. This will keep the paper from scorching. Iron the upper towel for about 3 to 5

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