Two adult C57BL/6 male and/or female black mice (20-30g) were Scheduled 1 euthanized by carbon dioxide asphyxiation and cervical dislocation according to the Animals Scientific Procedures Act 1986. The distal ileum (2-3cm) were dissected and mounted to the aerator, attached with a force transducer and placed in the organ bath (as shown in Figure 1) set between 32-37°C containing Krebs solution. Krebs solution was prepared making up 25L in volume with the following added in a large beaker with distilled water, continuously stirring in
