6. EXPERIMENAL WORK
6.1 Preliminary Work
6.1.1 Collection of Plant material
The leaves of Jasminum grandiflorum was collected locally in the month of July locally from Bhopal, Madhya Pradesh, India. Herbarium file of plant part was prepared and authenticated by Dr. Zia Ul Hasan (Professor, Department of Botany), Safia College Bhopal and the specimen voucher no.assigned was 344/Bot/safia/15
6.1.2 Drying and Size Reduction of Plant Material
The leaves of Jasminum grandiflorum was dried under shade in laboratory. They were pulverized to make coarse powder. The coarse powder of leaves was passed through sieve No. 16 to maintain uniformity and stored in cool and dry place for further study.
6.1.3 Screening of Powder (Physiochemical Analysis) …show more content…
No significant signs were noticed in animals. Hence administered dose was found tolerable as no death was found. Therefore, 2000mg/kg dose of extract of Jasminum grandiflorum was considered maximum safe dose.
6.6. In-Vivo pharmacological activity
Induction of diabetes: Diabetes mellitus was induced in rats by a single i.p. injection of alloxan monohydrate (120 mg/kg body weight) in normal saline (0.9% NaCl). Hyperglycemia was confirmed by fasting blood glucose level measurement by glucometer on the 3rd day after the alloxan injection. rats with consistent hyperglycemia on 3rd day (fasting blood glucose levels > 140 mg/dl) was considered diabetic and was used for further studies (Jyoti M, et al, 2002).
Experimental Design
In the experiment, a total of 30 rats were used. The rats were divided into 5 groups comprising of 6 animals in each group as follows: Group I: Normal control
6.1 Preliminary Work
6.1.1 Collection of Plant material
The leaves of Jasminum grandiflorum was collected locally in the month of July locally from Bhopal, Madhya Pradesh, India. Herbarium file of plant part was prepared and authenticated by Dr. Zia Ul Hasan (Professor, Department of Botany), Safia College Bhopal and the specimen voucher no.assigned was 344/Bot/safia/15
6.1.2 Drying and Size Reduction of Plant Material
The leaves of Jasminum grandiflorum was dried under shade in laboratory. They were pulverized to make coarse powder. The coarse powder of leaves was passed through sieve No. 16 to maintain uniformity and stored in cool and dry place for further study.
6.1.3 Screening of Powder (Physiochemical Analysis) …show more content…
No significant signs were noticed in animals. Hence administered dose was found tolerable as no death was found. Therefore, 2000mg/kg dose of extract of Jasminum grandiflorum was considered maximum safe dose.
6.6. In-Vivo pharmacological activity
Induction of diabetes: Diabetes mellitus was induced in rats by a single i.p. injection of alloxan monohydrate (120 mg/kg body weight) in normal saline (0.9% NaCl). Hyperglycemia was confirmed by fasting blood glucose level measurement by glucometer on the 3rd day after the alloxan injection. rats with consistent hyperglycemia on 3rd day (fasting blood glucose levels > 140 mg/dl) was considered diabetic and was used for further studies (Jyoti M, et al, 2002).
Experimental Design
In the experiment, a total of 30 rats were used. The rats were divided into 5 groups comprising of 6 animals in each group as follows: Group I: Normal control