The two solutions were combined in a microwave tube and put in the microwave for 30 minutes at 80oC. The solvent was evaporated under reduced…
The lab instructor issued out a test tube labeled with letter ‘I’, which consisted of two unknown bacteria, Gram-positive or Gram-negative that were streaked from a pure culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding contamination followed by rapidly flaming the neck of the test tube to prevent the entry and contamination of unwanted microbes.…
Interactive Question 7.2 Cite some experimental evidence that indicates that membrane proteins drift. A good form of experimental evidence is Fluorescence Recovery After Photobleaching, aka FRAP. In the FRAP process, membrane proteins are labelled with a green fluorescent protein, and part of the plasma membrane is “bleached” with a laser, causing them to lose their fluorescence. The part that was bleached will eventually become fluorescent again, as molecules drift in by diffusion.…
1.3.1 Measurement Overhead In this experiment, we found the latency incurred in measuring CPU cycles. This measurement of time overhead has to be subtracted from the forthcoming experiments. Methodology: We have used the RDTSC – RDTSCP instruction along with CPUID (serialize the instruction pipeline). This function fetches the count of CPU clock cycles.…
Results In the lab, 478 trials were conducted using a total of 956 crickets. The 478 pairs resulted in 264 wins for the resident cricket and 214 wins for the intruding crickets. These results produced a chi squared value of 5.230. The p-value calculated from this lab was .022.…
Materials. Numerous substances in the experiment were used. The most frequently used was the unknown due to the need to test its physical and chemical qualities. When a solution of the unknown was made, 1.000 g of the unknown and 1.0 mL of water was used to make it. To test for the possible ions, 1.0 mL of silver nitrate and 1.0 mL of nitric acid were used for the ion test.…
R.C. 5.9 Mathematics (Mobile Infant) Activity: “Stack Them Up” Materials: Stacking blocks, nesting cups, measuring cups Infants are continually growing in all areas of development. In this activity, the goal is to get the infant to stack their blocks or cups. The teacher would have the child sit on the floor or at a table with their toys.…
1. (8pts) From these data, graph your mean arterial pressure (MAP; see formula) and heart rate (y axes) vs. workload (in METS) on the x-axis (include resting results). Using this information, answer the following questions. Show ALL work. MAP…
The focus of this lab was to identify an unknown organism based on its characteristics and the results from each of the tests. There will be various of test to choose from in order to identify the unknown organism, which will eliminate numerous possibilities and narrow it down to one. All the fundamental skills that we have learned and practiced in the lab will be used to perform on our unknown such as aseptic technique, microscopic examination, the use of differential media, and determining if it’s positive or negative. Performing aseptic techniques is the most crucial step that requires the utilizing of transferring, inoculating, and storing bacterial cultures and media. Aseptic technique is defined as procedures that prevent contamination…
In particular, there was a significant amount of drying agent added which may have resulted in the loss of some product. As well, some product was lost in the removal of the product from the drying agent and during the distillation because of incomplete transfer. In addition, some was also likely lost through draining along with the aqueous phase during the washes and extractions. Improving the efficiency of these could increase the yield of the product by causing less…
In part two of this experiment the myristic acid is prepared from the trimyristin through hydrolysis. The experimenter’s objective in this part of the experiment is to synthesize myristic acid from the trimyristin in the first part of the experiment. In order to complete this, base promoted hydrolysis of an ester is used. The crude myristic acid is purified through recrystallization and then compared to the literature melting point. Sodium hydroxide is a strong base that can be added to fats or oils when heated.…
The first being that sulfuric acid acts as a catalyst that helps speed up esterification at room temperature. The second reason being that sulfuric acid is a dehydrating agent. Since water is a product in esterification, sulfuric acid will force the equilibrium to the right, resulting in a higher yield of the ester. To determine the unknown alcohols used in producing the esters, retrosynthetic analysis was performed by working backwards and visualizing the bonds that were formed in order to create that ester. There were several possibilities for error that accounted for our low percent yield (70.7%) of acetaminophen.…
Gel electrophoresis is a method used for separation and analysis of molecules such as DNA, RNA, and proteins, based on their sizes and polarity. DNA (deoxyribonucleic acid) is a molecule that carries most of our genetic information, and possesses a negative charge. During gel electrophoresis, DNA fragments can migrate through the gel also known as agarose when placed in a powerful electrical field. The rate at which the DNA fragments will move through the gel depends on their relative size. Horizontal gel slabs are commonly used on conducting gel electrophoresis.…
Paracetamol is one of the world’s most beneficial and resourceful analgesic and is widely used in present day. An analgesic, can be defined, as a chemical compound that relieves pain, reduces fever and inflammation. It works by hindering the enzyme cyclooxygenase which acts a catalyst to convert fatty acids to prostaglandins. Prostaglandins cause pain and inflammation to peripheral and central points in the nervous system2. Paracetamol, or Acetaminophen is created by the synthesis and preparation of an amide.…
[Chemical Engineering Lab Practice 4] EXTRACTION OF BENZOIC ACID Candidate’s Name: Raajeswari A/P Radahakrishnan Student ID: SCM-023562 Group Member’s Name: Angela A/P Gunasegaran Shubashinee A/P Murugan Lecturer/ Supervisor: Ms Nazlina & Dr Ong Chi Siang…