The objective of the present study is to isolate piperine from the black pepper. Our attempt was to extract the piperine with petroleum ether from black pepper and isolation using the same solvent. It is very difficult to extract and isolate the piperine using a non-polar solvent like petroleum ether due to solubility. During our study, we observe that piperine have a very little solubility in the petroleum ether, at the same time the solubility of the resinous material in petroleum ether is also less. But comparatively, it has more solubility than piperine. Generally, non-polar solvents like petroleum ether were used to extract the oil from the plant material and polar solvents are used to extract polar molecules like Piperine. In our study,
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So we tried to extract and isolate pipeine at RT. Generally, ratio of liquid to raw material on the extraction is an important factor which is to be considered and studied. Use of large solvent volume increases the extraction efficiency, but at the same time results in unnecessary wasting of solvent and low liquid –solid ratio will results in incomplete extraction. We extracted Black pepper with 10 X 30 ml of petroleum ether. After concentration to minimum volume, it gave solid piperine. The results are listed in the table no. 1. From the results, it observed that the amount of piperine isolated is only 0.8%. That means large amount of piperine is not extracted and it need to extract further number of times, but very important achievement is that piperine is isolated without any further …show more content…
The results are listed in the table 2. From the result, it is observed that isolation of piperine at 40°c results in the free solid. If the isolation performed at 25°c or at lower temperature then the oily material also fallout and it results in the sticky mass. It can also tackled by washing the solid with cold diethyl ether (DEE), but it results in the loss of piperine. The yield obtained during this process was around 3.0%.
The yield of crude piperine by the proposed method was found to be 3.0% However on purification, it was found to be 2.4%. The melting point of isolated compound was found to be 131-132 ºc which is matching with the reported melting point in literature[8]. And the IR spectrum of the isolated compound was also found to matching with the reported IR spectrum in literature[8].
Spectrophotometric Analysis:
The piperine content of the pepper was found by Spectrophotometric analysis method[10,11]. The Spectrophotometric analysis was performed on UV-Visible Spectrophotometer Instrument (UV-1800, Shimadzu). The piperine content of the pepper was found to be
So we tried to extract and isolate pipeine at RT. Generally, ratio of liquid to raw material on the extraction is an important factor which is to be considered and studied. Use of large solvent volume increases the extraction efficiency, but at the same time results in unnecessary wasting of solvent and low liquid –solid ratio will results in incomplete extraction. We extracted Black pepper with 10 X 30 ml of petroleum ether. After concentration to minimum volume, it gave solid piperine. The results are listed in the table no. 1. From the results, it observed that the amount of piperine isolated is only 0.8%. That means large amount of piperine is not extracted and it need to extract further number of times, but very important achievement is that piperine is isolated without any further …show more content…
The results are listed in the table 2. From the result, it is observed that isolation of piperine at 40°c results in the free solid. If the isolation performed at 25°c or at lower temperature then the oily material also fallout and it results in the sticky mass. It can also tackled by washing the solid with cold diethyl ether (DEE), but it results in the loss of piperine. The yield obtained during this process was around 3.0%.
The yield of crude piperine by the proposed method was found to be 3.0% However on purification, it was found to be 2.4%. The melting point of isolated compound was found to be 131-132 ºc which is matching with the reported melting point in literature[8]. And the IR spectrum of the isolated compound was also found to matching with the reported IR spectrum in literature[8].
Spectrophotometric Analysis:
The piperine content of the pepper was found by Spectrophotometric analysis method[10,11]. The Spectrophotometric analysis was performed on UV-Visible Spectrophotometer Instrument (UV-1800, Shimadzu). The piperine content of the pepper was found to be