Temperature and pH will affect enzyme function by increasing the reaction rate because as temperature increase so is the rate of reaction and extremely high or low pH will result in the denatured by decreasing the reaction rate. The function of this experiment was to investigate the influence of the concentration of enzyme on the rate of reaction. The rate of reaction in this experiment was subjected by the amount of Guaiacol, H2O2, Extracts, and Buffer (pH5). The reaction rates with different enzyme concentration at 0.5x (ml) was slow but gradually increase making the absorbance below 0.1NM. At 1.0x (ml) the rate was a little slow but then it increased rapidly. The reaction rate as observed at 2.0x (ml) went immediately up in the absorbance
