In this lab we learned the basics of enzymes and how they work. We were able to perform a quantitative assay of the activity of an enzyme in a tissue extract using a spectrophotometer. Also, we had to organize the data that was provided into tables and graphs so we could have the ability to test a few hypotheses. For example, we tested the whether the rate of the reaction was influenced by enzyme concentration, whether the activity of the enzyme was influenced by temperature and also too see if the activity of the enzyme was influenced by the pH of the solution, which you will then see the results on the graphs and charts that I provided. In figure one we show results of the trial run from the enzyme activity where the absorbance was at 500nm. Both the chart and graph show us the change between time, but most importantly it shows us how the absorbance rates seem to gradually increase within time. In the second figure, we provide information about the reaction rate vs. concentration. In this chart we can see the numbers increase but then decrease a little, which means that the reaction rate is not consistent. In figure three I provide the information about the effects of temperature on absorbance vs. time. In this figure we can see the number slightly increase throughout the different temperatures, it almost seems as if the temperature doesn’t really have an effect on the absorbance rate, but it slightly does.
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Now, the fourth figure provides information based on the reaction rate vs. time. Out of all of the figures provided I think this is the one that actually shows drastic change. For example, we are able to see how the reaction rate vs. temperature starts off high and increase gradually for a little bit and then it decreases drastically. It seems as the temperature increases the reaction rate slows down each time. Then, in figure five I am able to provide information regarding the absorbance vs. time for pH. In this figure we are able to see that all of the absorbance rates start at the same level and then gradually increase, except for the one with the level of pH 9. PH 9 is the only rate in this figure that increases and then decreases drastically only to increase again. This can show us that although a couple of absorbance levels might drop but will increase again. Lastly, in figure 6 I am able to provide information regarding the reaction rate vs. pH. Both the graph and chart show us that the reaction rate increases rapidly at first and then it begins to gradually drop. This shows us that as the pH levels change the reaction rate is likely to decrease. The results of our experiment reflected our initial hypothesis because we concluded that whenever the concentration rate increase so would the reaction rate but we were wrong because although it did increase it wasn’t consistent due to the fact that it decreased. When it came to the temperature we also concluded that once it increased so would the absorbance rate and although it is not increasing drastically it is still increasing slowly. It almost seems as if the temperature doesn’t have an effect on it since the change isn’t really noticeable. When it came to making a hypothesis for how the pH levels would affect the absorbance rate we weren’t quite
Now, the fourth figure provides information based on the reaction rate vs. time. Out of all of the figures provided I think this is the one that actually shows drastic change. For example, we are able to see how the reaction rate vs. temperature starts off high and increase gradually for a little bit and then it decreases drastically. It seems as the temperature increases the reaction rate slows down each time. Then, in figure five I am able to provide information regarding the absorbance vs. time for pH. In this figure we are able to see that all of the absorbance rates start at the same level and then gradually increase, except for the one with the level of pH 9. PH 9 is the only rate in this figure that increases and then decreases drastically only to increase again. This can show us that although a couple of absorbance levels might drop but will increase again. Lastly, in figure 6 I am able to provide information regarding the reaction rate vs. pH. Both the graph and chart show us that the reaction rate increases rapidly at first and then it begins to gradually drop. This shows us that as the pH levels change the reaction rate is likely to decrease. The results of our experiment reflected our initial hypothesis because we concluded that whenever the concentration rate increase so would the reaction rate but we were wrong because although it did increase it wasn’t consistent due to the fact that it decreased. When it came to the temperature we also concluded that once it increased so would the absorbance rate and although it is not increasing drastically it is still increasing slowly. It almost seems as if the temperature doesn’t have an effect on it since the change isn’t really noticeable. When it came to making a hypothesis for how the pH levels would affect the absorbance rate we weren’t quite