Egg Cell Essay

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-In a SCNT, the nucleus of an egg cell is removed and it becomes an enucleated egg cell.
-The nucleus is removed because the nucleus contains the majority of the genetic information (DNA).
-DNA has a double helix structure and it is made from many nucleotides
-Nucleotides are made from a nitrogen base (Adenine, Guanine, Cytosine, or Thymine), a phosphate group, and a deoxyribose.
-The DNA is stored in the nucleus as chromatin, but during cell division the DNA stored as chromatin condense into chromosomes.
-The DNA initially condenses into chromatin when it is wrapped around histone (a protein molecule) and then coiled.
-Histone is positively charged (DNA is negatively charged) and is made from four main proteins.
-Once DNA is wrapped into chromatin, it is further coiled and condensed to make chromosomes.
-In
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-mtDNA contains about 16,00 DNA base pairs (37 genes).
- mtDNA codes for enzymes that help make ATP from oxygen and sugar (the process is called oxidative phosphorylation) and also codes for tRNA and rRNA.
-The majority of the mitochondrial DNA can be eliminated through the use of ethidium bromide.
-If a SCNT ‘clone’ is homoplasmic, it would still not be a true clone because it would have the mt genome of the original egg cell (to be a true clone it would have to have all of it’s DNA come from the somatic cell). -After the somatic nucleus is inserted into the egg cell, the egg cell behaves as if it was fertilized and begins to divide.
-Once there are about 100 egg cells, a blastocyst forms (an early embryo).
-An embryo is a multicellular organism in its early stages of development.
-The blastocyst is ‘genetically identical’ (except for the mtDNA) to the somatic nucleus that was transferred into the cell.
-This blastocyst can then be used for its stem cells (therapeutic cloning) or inserted into an uterus (reproductive cloning).
-If the blastocyst is inserted in an uterus, it eventually will develop into a

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