Effect of Temperature and Substrate Concentration on Catalase Reactions
Blake Devivo
Biol 101 Lab
February 10, 2016
Abstract The human body contains thousands of tiny structures called enzymes that perform specific functions. One enzyme called “Catalase” is often used to regulate the amount of a toxic compound, hydrogen peroxide, in the body. The purpose of this experiment was find the different factors that can affect Catalase-Peroxide reactions. The first part of this experiment demonstrated that catalase reactions with 0.8% peroxide produce 57±4.75 mL of O2 per minute while those with 0.1% peroxide produce 0.7±0.20 mL of 02 per minute (p=0.0000… ) . The second part of this experiment demonstrated that catalase peroxide reactions at …show more content…
Error bars represent SEM
Experiment Catalase Treatment t-test matchup p-value
Effect of varying substrate concentration Regular Catalase (phosphate buffer, pH 6.8) 0% vs 0.1%
0.1% vs 0.2%
0.2% vs 0.4%
0.4% vs 0.8% p= 0.002518333
p= 0.000243814
p= 1.35676E-05
p= 0.000258456
Table 1 – Statistics table for average reaction rate for varying substrate concentrations
In the second part of the experiment, there was a steady increase in reaction rate with temperature (see Table 2) up to 37°C. The pre-boiled catalase did not react to produce any 02. Three two-sample t-test were performed between the boiled and cold reaction rate (p = 0.000112721), the cold and room temperature reaction rate (p = 0.000243814), and the room temperature and warm reaction rate p = 0.447281352. The data are represented graphically in Figure 2 and Table 2.
Figure 2 – Average reaction rate with increasing temperatures. Error bars represent SEM
Experiment Catalase Treatment t-test matchup p-value
Effect of varying temperature Regular Catalase (phosphate buffer, pH 6.8) Boiled vs Cold
Cold vs Room Temp
Room Temp vs Warm p= 0.000112721
p= …show more content…
The data revealed a clear direct relationship between temperature and reaction rate. When the reaction rates of the three temperatures were compared with a t-test in order, most of them returned a p-value below the 0.05 threshold. This means that there was a significant increase in reaction rate each time the temperature increased, except for in the boiled catalase, which showed no reaction. have rendered the catalase useless. These results are consistent with our hypothesis and previously researched data that state that reaction rates are increased within a range of temperatures. One limitation in this experiment is the difficulty in consistently keeping catalase at temperatures different from room temperature. Another possible source of error could come from contamination build up from using the same glassware in repeated trials. If repeated, this experiment could be done better by carefully monitoring the temperature of samples, as well as by though roughly cleaning glassware between the
Blake Devivo
Biol 101 Lab
February 10, 2016
Abstract The human body contains thousands of tiny structures called enzymes that perform specific functions. One enzyme called “Catalase” is often used to regulate the amount of a toxic compound, hydrogen peroxide, in the body. The purpose of this experiment was find the different factors that can affect Catalase-Peroxide reactions. The first part of this experiment demonstrated that catalase reactions with 0.8% peroxide produce 57±4.75 mL of O2 per minute while those with 0.1% peroxide produce 0.7±0.20 mL of 02 per minute (p=0.0000… ) . The second part of this experiment demonstrated that catalase peroxide reactions at …show more content…
Error bars represent SEM
Experiment Catalase Treatment t-test matchup p-value
Effect of varying substrate concentration Regular Catalase (phosphate buffer, pH 6.8) 0% vs 0.1%
0.1% vs 0.2%
0.2% vs 0.4%
0.4% vs 0.8% p= 0.002518333
p= 0.000243814
p= 1.35676E-05
p= 0.000258456
Table 1 – Statistics table for average reaction rate for varying substrate concentrations
In the second part of the experiment, there was a steady increase in reaction rate with temperature (see Table 2) up to 37°C. The pre-boiled catalase did not react to produce any 02. Three two-sample t-test were performed between the boiled and cold reaction rate (p = 0.000112721), the cold and room temperature reaction rate (p = 0.000243814), and the room temperature and warm reaction rate p = 0.447281352. The data are represented graphically in Figure 2 and Table 2.
Figure 2 – Average reaction rate with increasing temperatures. Error bars represent SEM
Experiment Catalase Treatment t-test matchup p-value
Effect of varying temperature Regular Catalase (phosphate buffer, pH 6.8) Boiled vs Cold
Cold vs Room Temp
Room Temp vs Warm p= 0.000112721
p= …show more content…
The data revealed a clear direct relationship between temperature and reaction rate. When the reaction rates of the three temperatures were compared with a t-test in order, most of them returned a p-value below the 0.05 threshold. This means that there was a significant increase in reaction rate each time the temperature increased, except for in the boiled catalase, which showed no reaction. have rendered the catalase useless. These results are consistent with our hypothesis and previously researched data that state that reaction rates are increased within a range of temperatures. One limitation in this experiment is the difficulty in consistently keeping catalase at temperatures different from room temperature. Another possible source of error could come from contamination build up from using the same glassware in repeated trials. If repeated, this experiment could be done better by carefully monitoring the temperature of samples, as well as by though roughly cleaning glassware between the