For the other agar plates, here is what should have been seen: the agar plate containing the pUC18 plasmid (Fig 2: top left) should have had similar results to the agar plate containing the lux plasmid (Fig 2: middle right), meaning there should have been both lawn and colonial growth, minus the chance of bioluminescence. When in reality, the agar plates taken from the experiment did reveal colonial growth but for the wrong reason. The growth seen in this agar plate (Fig 2: top left) was of another type of bacteria, possibly airborne bacteria that could have fallen onto the agar gel while the experiment was conducted. A possible explanation for why there was no growth of the bacterium E. coli could have been that the cell spreader could have been so hot that it killed all of the E. coli cells placed onto the agar gel and destroyed any chance of growth for this particular
For the other agar plates, here is what should have been seen: the agar plate containing the pUC18 plasmid (Fig 2: top left) should have had similar results to the agar plate containing the lux plasmid (Fig 2: middle right), meaning there should have been both lawn and colonial growth, minus the chance of bioluminescence. When in reality, the agar plates taken from the experiment did reveal colonial growth but for the wrong reason. The growth seen in this agar plate (Fig 2: top left) was of another type of bacteria, possibly airborne bacteria that could have fallen onto the agar gel while the experiment was conducted. A possible explanation for why there was no growth of the bacterium E. coli could have been that the cell spreader could have been so hot that it killed all of the E. coli cells placed onto the agar gel and destroyed any chance of growth for this particular