CAUTION:
It is best not to get dichloromethane on your skin. Gloves are to be worn during this experiment, if improper safety precautions are taken there is a possibility of irritation, and or burning of the skin.
CAUTION:
Gas will build up in the separatory funnel while you are shaking it, so you must vent the funnel occasionally to prevent the stopper from popping out, causing a mess and possibly getting dichloromethane on your skin.
~During the pre-phase of this experiment it is recommended that you prepare all aspects of this experiment ensuring any safety reliability’s. It is recommended that you have chosen a clean safe environment to perform this experiment. All actions taken place during this experiment are under your care and will reflect the outcome of this experiment, and are under no circumstances my responsibilities. Starting off fill a two hundred and fifty (250) milliliter beaker with ten (10) grams of coffee, then add four (4) grams of Sodium Carbonate. The Sodium Carbonate will react with a lot of molecules in the coffee making them more water soluble. To the coffee and Sodium Carbonate is then added sixty (60) milliliters of distilled water, a watch glass is to be placed on top, and then the beaker transferred to a hot plate where it is brought to a boil. The solution has a tendency to foam over so to confiscate for this use a glass stirring rod and stir the mixture. Foaming occurs when too much heat is applied to the solution, to remedy this simply turn down the hotplate. After fifteen (15) minutes remove the solution from the hot plate and attach a coffee filter by a rubber band to the top of a four hundred (400) milliliter beaker. It is then you pour the solution from the two hundred fifty (250) milliliter beaker to the four hundred (400) milliliter beaker to remove the coffee grinds from the solution. To limit some of the heat loss while the solution is filtering a watch glass is to be placed on top. Once all the coffee has filtered through thirty (30) milliliters of boiling distilled water is used to clean out the original two hundred and fifty (250) milliliter beaker, and is then pour into the filter. The watch glass is to be placed over the beaker once more. After it is filtered through once again, an extra one hundred (100) milliliters of boiling distilled water is added and allowed to filter though. Again a watch glass is placed on top, and the beaker is moved to a hot place and set on low (to ensure the temperature of the coffee). What left after all the filtration steps is basically a bunch of strong coffee. The coffee is then transferred to a seperatory funnel …show more content…
Add twenty (20) milliliters of dichloromethane and place the stopper in the top of the sep funnel. Shake the funnel for 1-2 minutes being care not to spill its contents. Place the funnel back in the ring stand and allow the two layers of liquid to separate—this may take a few minutes. The lower layer will be dichloromethane. There may be an emulsion at the boundary between the two layers—an emulsion is a region where “bubbles” of one liquid are suspended in the other liquid, and the two refuse to separate. If an emulsion occurs, use a glass stirring rod to poke around in the emulsion to break it up. Be sure the stopper is removed from the top of the sep funnel for the following drainage step—otherwise the liquid will drain out of the funnel extremely slowly. Once the two layers have separated, or are nearly separated, drain the lower dichloromethane layer into a clean, DRY two hundred and fifty (250) milliliter beaker. Be careful not to drain any of the coffee into the beaker. It is best to leave a very small amount of dichloromethane at the bottom of the seperatory funnel so as not to accidently allow some coffee to escape. The dichloromethane just drained contains dissolved caffeine that has been extracted from the coffee. However, it isn’t possible to extract all the caffeine with a single portion of dichloromethane. Therefore, you will do two more extractions using fresh portions of dichloromethane in order to extract the majority of the caffeine that is in the coffee. Set the beaker containing the first portion of dichloromethane
It is best not to get dichloromethane on your skin. Gloves are to be worn during this experiment, if improper safety precautions are taken there is a possibility of irritation, and or burning of the skin.
CAUTION:
Gas will build up in the separatory funnel while you are shaking it, so you must vent the funnel occasionally to prevent the stopper from popping out, causing a mess and possibly getting dichloromethane on your skin.
~During the pre-phase of this experiment it is recommended that you prepare all aspects of this experiment ensuring any safety reliability’s. It is recommended that you have chosen a clean safe environment to perform this experiment. All actions taken place during this experiment are under your care and will reflect the outcome of this experiment, and are under no circumstances my responsibilities. Starting off fill a two hundred and fifty (250) milliliter beaker with ten (10) grams of coffee, then add four (4) grams of Sodium Carbonate. The Sodium Carbonate will react with a lot of molecules in the coffee making them more water soluble. To the coffee and Sodium Carbonate is then added sixty (60) milliliters of distilled water, a watch glass is to be placed on top, and then the beaker transferred to a hot plate where it is brought to a boil. The solution has a tendency to foam over so to confiscate for this use a glass stirring rod and stir the mixture. Foaming occurs when too much heat is applied to the solution, to remedy this simply turn down the hotplate. After fifteen (15) minutes remove the solution from the hot plate and attach a coffee filter by a rubber band to the top of a four hundred (400) milliliter beaker. It is then you pour the solution from the two hundred fifty (250) milliliter beaker to the four hundred (400) milliliter beaker to remove the coffee grinds from the solution. To limit some of the heat loss while the solution is filtering a watch glass is to be placed on top. Once all the coffee has filtered through thirty (30) milliliters of boiling distilled water is used to clean out the original two hundred and fifty (250) milliliter beaker, and is then pour into the filter. The watch glass is to be placed over the beaker once more. After it is filtered through once again, an extra one hundred (100) milliliters of boiling distilled water is added and allowed to filter though. Again a watch glass is placed on top, and the beaker is moved to a hot place and set on low (to ensure the temperature of the coffee). What left after all the filtration steps is basically a bunch of strong coffee. The coffee is then transferred to a seperatory funnel …show more content…
Add twenty (20) milliliters of dichloromethane and place the stopper in the top of the sep funnel. Shake the funnel for 1-2 minutes being care not to spill its contents. Place the funnel back in the ring stand and allow the two layers of liquid to separate—this may take a few minutes. The lower layer will be dichloromethane. There may be an emulsion at the boundary between the two layers—an emulsion is a region where “bubbles” of one liquid are suspended in the other liquid, and the two refuse to separate. If an emulsion occurs, use a glass stirring rod to poke around in the emulsion to break it up. Be sure the stopper is removed from the top of the sep funnel for the following drainage step—otherwise the liquid will drain out of the funnel extremely slowly. Once the two layers have separated, or are nearly separated, drain the lower dichloromethane layer into a clean, DRY two hundred and fifty (250) milliliter beaker. Be careful not to drain any of the coffee into the beaker. It is best to leave a very small amount of dichloromethane at the bottom of the seperatory funnel so as not to accidently allow some coffee to escape. The dichloromethane just drained contains dissolved caffeine that has been extracted from the coffee. However, it isn’t possible to extract all the caffeine with a single portion of dichloromethane. Therefore, you will do two more extractions using fresh portions of dichloromethane in order to extract the majority of the caffeine that is in the coffee. Set the beaker containing the first portion of dichloromethane