Gram negative bacteria are more susceptible to heat, because they do not have a thick peptidogylcan layer like gram positive bacteria does. Gram negative bacteria has a outer membrane of LPS, that is affected by heat easier.
The five
M.Luteus is known for its extremely low virulence, and it is usually considered…
In order to determine this I performed a gram stain on each of the bacteria. I observed each bacterial slide on a microscope under 1000X resolution. The bacterium labeled 7A showed multiple staphylococcus arrangements that were stained red. I determined that this bacterium was gram negative because of red color of the bacteria. The bacterium labeled 7B showed multiple single bacilli that were stained purple.…
Gram Negative Unknown Lab Report #20 BIOL 3444-008 Giang Nguyen TA: Malini Kotak 11/05/2014 Abstract The experiment is conducted in order to identify a randomly given unknown gram-negative microorganism. By comparative analysis of biochemical tests that were performed throughout the semester to find out its characteristics and determine the identification of bacterium out of four potential unknown was given. To figure out the characteristics, T-streak was performed to isolate the sample from broth to a Tryptic Soy Agar plate, along with an additional slant in case something wrong with the original plate that can possibly affect the result.…
Investigating Enterococcus faecalis’s structures and resistances to antibiotics Introduction Bacteria’s phenotype is commonly described as gram-negative or gram-positive from conducting a gram stain. A bacterium that is gram-negative tends to have cells composed of a thin peptidoglycan layer, and a gram stain of gram-negative bacteria are seen as red or pink through a microscope (Smith and Hussey, 2005). On the other hand, a bacterium that is gram-positive will have cells made of a thick peptidoglycan layer, and the gram stain of a gram negative bacteria would be seen blue or purple (Smith and Hussey, 2005). In particular, Enterococcus faecalis is a gram-positive bacterium which has been found in many infections in the urinary tract, surgical,…
A sample is taken from the broth as well as the streak plate using a sterile loop and heat-fixed onto a clean slide. The slide is then stained using the gram-stain technique to determine whether the bacteria is positive or negative based on the chemical and physical properties of the bacteria's cell wall. After observation, the bacteria were determined to be gram negative since they had stained red. It was also observed that the bacteria were…
The study of microbiology requires not only understanding the microscopic organisms, but also the understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. The identification of microorganisms is not only important in microbiology lab, but also in the medical field to identify an agent of a disease that will help treat the patient by using the correct antibiotics to kill off the host. In this unknown lab report, techniques and procedures learned in the microbiology laboratory during the semester that was performed to test ones practical understanding of microbiology. The sole purpose of the unknown lab is to demonstrate understanding of the experimental methods and lab techniques learned during…
The inability to clear trapped bacteria in the mucous promotes microbial colonization and damages the lungs causing poor pulmonary function. Pseudomonas aeruginosa is also known to be a common causative agent of wound infections. These wound infections are more common in people with weaker immune systems, such as those with diabetes mellitus, as it causes wounds to heal at a slower rate which makes them prone to infections. P. aeruginosa wound infections occur most often in the hospital from surgical wound treatment and are known to be the leading cause of burn infections (Nedeljković, Tiodorović, Kocić, Cirić, Milojković, and Waisi…
Introduction: The world of antiseptics and disinfectants is ever changing. There are many different options out there and people have to decide what is the best option for them. For the experiment we performed in class, we had a variety of different options to use to see what the results would be from these. The range of items used were from and everyday household product of Lysol to Iodine which can be used in the operating room to prep the patient for the type of surgery they are having done. The purpose of this experiment was to show how effective products such as Lysol and Hibiclens can be against different bacteria.…
Experimental trials were performed by students over a timeframe of three weeks to determine the identity of three unknown bacteria contained in broth medium stored in labeled test tubes that were randomly assigned containing: one Gram positive bacterium, one Gram-negative paracolon, and one Gram-negative coliform (Carson, 2016). The unknown sample assigned in this experiment was #19. After initially streaking the solution on clean streak plates and TSA agar, the identity was determined from a Gram-stain of a single, isolated colony; this was confirmed by isolation of that pure colony and viewing the pure sample in a Gram-stain under a microscope yielding a blue colored cocci morphology with no Gram-negative contamination. This was followed by Gram-negative isolation by a Gram-stain showing a myriad of Gram-negative bacillus rods, with no presence of Gram-positive. Further tests…
Gram-negative bacteria are often harmful to a host, which is the case for many of the Salmonella bacteria. Protists 1.) Irish Moss- I knew that this was Irish Moss because it was reddish and kind of like seaweed. This is a protist because it lives in the water like a regular protist would normally do.…
Some strains aid and prevent the growth of other bacteria in the body. Unfortunately, other strains of the same bacteria have evolved to cause…
Bacteria can be classified as Gram positive or negative (difference in cell wall), with the help of the Gam stain (“Gram Stain”, n. d). Another way to help differentiate a specific bacterium is to utilize biochemical tests. Biochemical test is used to indicate if the bacteria can metabolize a certain ingredient. For example, the phenol red broths are three different kind…
In 1969, Thomas Brock and Hudson Freeze of Indiana University discovered a new bacteria species in the Yellow Stone Park, that scientist thought it could not survive above 55 degrees Celsius. Thermus aquaticus is a typical gram-negative bacterium, meaning that its cell walls contain much less peptidoglycan and unlike gram-positive bacteria, gram-negative bacteria contain lipoproteins. T. aquaticus appears as either a rod or short filaments. When exposed to sunlight, it can exhibit a yellow, pink, or red color due to pigments within the bacteria. Along with the coloration, T. aquaticus can either have flagella or be immotile.…
It is predominantly viable against Mycoplasma spp., Pasteurella spp., and many Gram-positive bacteria with viability against anaerobic bacteria and some Gram-negative respiratory bacteria such as Pasteurella multocida and Mannheimia…
Introduction: The effectiveness of Antimicrobial agents against Microorganisms Population of microorganisms or growth are killed and/or inhibited by natural or synthetic chemical antimicrobial controls (Madigan, et al 2012). Antimicrobial agents are grouped according to their efficacy against microorganisms. Bactericidal that kill and bacteriostatic prevent and/or reduce growth (Madigan et al, 2012).…