Clostridium perfringens is typically found in soil and if this is eaten or coming in contact with the animal it can cause variety of diseases, especially in domestic animals. These diseases include different enteric syndromes such as bovine and ovine enterotoxemia, fowl necrotic enteritis, and lamb dysentery. Strains of most toxin types like alpha and beta toxin have been involved in necrotic enteritis beta toxin is caused by type-C strains. The pulpy kidney disease is caused by type-D strains of Clostridium perfringens and this is associated to protein, a low to a high protein feed. (Rood J. I., 1998)
In poultry Clostridium perfringens is the causative agent of necrotic enteritis. Clostridium perfringens type A has been found in diseased …show more content…
Clostridium perfringens in adult cattle is referred to as a Hemorrhagic Bowel, Jejunal Hemorrhage Syndrome (JHS) or Bloody Gut. Clostridium perfringens type A is believed to be the causative agent of JHS. These diseases that affect cow is characterized by little or no milk, little manure and can die even if surgical intervention is given. Cattles that are affected with JHS has the high risk factor of some of the …show more content…
In egg-yolk agar the colony will look opaque, and whitish precipitate is formed within the media.
(Quinn, Carter, Markey, & Carter, 1994) stated that for isolation, there should be a freshly prepared or pre-reduced blood agar. Inoculated blood and MacConkey agar plate will be incubated aerobically. These plates will discover aerobic pathogens if present and used to avoid contamination of the specimen. Cooked meat broth and thioglycollate medium a liquid or semi-solid medium with low redox potential can be used for growth and maintenance of pure culture. Boiling to expel absorbed oxygen should be undertaken before inoculating the cooked meat broth, followed by rapid cooling to 37 degrees Celsius.
BACTERIAL MORPHOLOGY AND ANATOMY (Merchant & Packer, 1967) States that it is rod in shape, diameter is 0.8μ to 1.5μ, length is 4μ to 8μ and usually the shape of the ends is square and in the book of (Timoney, Gillespie, & Barlough, 2008) states that the cell is 1 μ wide and 4 to 8 μ long. Under settled growth conditions we can see short, almost coccal forms and long filaments. In old coagulated serum cultures we can often found club shapes, filaments, tadpole forms and granular types. Arrangement is usually singly but sometimes found in pairs, short chains and in chains or filaments.
In poultry Clostridium perfringens is the causative agent of necrotic enteritis. Clostridium perfringens type A has been found in diseased …show more content…
Clostridium perfringens in adult cattle is referred to as a Hemorrhagic Bowel, Jejunal Hemorrhage Syndrome (JHS) or Bloody Gut. Clostridium perfringens type A is believed to be the causative agent of JHS. These diseases that affect cow is characterized by little or no milk, little manure and can die even if surgical intervention is given. Cattles that are affected with JHS has the high risk factor of some of the …show more content…
In egg-yolk agar the colony will look opaque, and whitish precipitate is formed within the media.
(Quinn, Carter, Markey, & Carter, 1994) stated that for isolation, there should be a freshly prepared or pre-reduced blood agar. Inoculated blood and MacConkey agar plate will be incubated aerobically. These plates will discover aerobic pathogens if present and used to avoid contamination of the specimen. Cooked meat broth and thioglycollate medium a liquid or semi-solid medium with low redox potential can be used for growth and maintenance of pure culture. Boiling to expel absorbed oxygen should be undertaken before inoculating the cooked meat broth, followed by rapid cooling to 37 degrees Celsius.
BACTERIAL MORPHOLOGY AND ANATOMY (Merchant & Packer, 1967) States that it is rod in shape, diameter is 0.8μ to 1.5μ, length is 4μ to 8μ and usually the shape of the ends is square and in the book of (Timoney, Gillespie, & Barlough, 2008) states that the cell is 1 μ wide and 4 to 8 μ long. Under settled growth conditions we can see short, almost coccal forms and long filaments. In old coagulated serum cultures we can often found club shapes, filaments, tadpole forms and granular types. Arrangement is usually singly but sometimes found in pairs, short chains and in chains or filaments.