Benzimidazole and its derivatives were synthesized according to the reported literature (Wagner EC et al., 1943). The compounds are benzimidazole ;1-(4-((1H-benzimidazol-1-yl) methylamino) phenyl) ethanone; 2-butyl-1H-benzimidazole; 2-methyl-1H-benzimidazole. The stock solutions are prepared by dissolving the compound in DMSO. Antimicrobial activity was tested by the disc-diffusion method under standard conditions using Mueller-Hinton Agar (MHA) and the antibiotics used as listed below, Table 1.As control MTCC K. pneumoniae 7407, MTCC S. aureus 3160 and ATCC E.coliJ53.
Antimicrobial disc diffusion susceptibility
AST was done by Disc-Diffusion method (Qureshi AH et al., 2004) for all the derivatives at the concentration 1mg/ml to find out the zone of Inhibition. The derivatives of different concentration were added to the 9mm sterilized filter paper disc and placed on the culture swabbed MHA plates. The diameters of the zone of inhibition were measured in millimetres. …show more content…
Andrews, 2011). As a control stock antibiotic concentration of Ampicillin (Hi-Media) was prepared by diluting 1mg in 1 ml of autoclaved distilled water and stored at 4°C. The Working concentration used was 1000μg/1ml of DMSO. Finally the MIC value was converted to mL
GC- MASS SPECTROMETER
The JEOL GCMATE II GC-MS with Data system is a high resolution, double focusing instrument. Maximum resolution: 6000 Maximum calibrated mass: 1500 Daltons. Source options: Electron impact (EI); Chemical ionization (CI) (https://saif.iitm.ac.in/newjeol.html).
MOLECULAR MODEL AND DOCKING
Antimicrobial disc diffusion susceptibility
AST was done by Disc-Diffusion method (Qureshi AH et al., 2004) for all the derivatives at the concentration 1mg/ml to find out the zone of Inhibition. The derivatives of different concentration were added to the 9mm sterilized filter paper disc and placed on the culture swabbed MHA plates. The diameters of the zone of inhibition were measured in millimetres. …show more content…
Andrews, 2011). As a control stock antibiotic concentration of Ampicillin (Hi-Media) was prepared by diluting 1mg in 1 ml of autoclaved distilled water and stored at 4°C. The Working concentration used was 1000μg/1ml of DMSO. Finally the MIC value was converted to mL
GC- MASS SPECTROMETER
The JEOL GCMATE II GC-MS with Data system is a high resolution, double focusing instrument. Maximum resolution: 6000 Maximum calibrated mass: 1500 Daltons. Source options: Electron impact (EI); Chemical ionization (CI) (https://saif.iitm.ac.in/newjeol.html).
MOLECULAR MODEL AND DOCKING