B7 Lab Report

Day 1: On the first day of testing the unknown bacteria, B7 was aseptically inoculated in four different ways. The first procedures that were done was the inoculation of a nutrient broth tube, nutrient agar slant, and a nutrient agar plate. These three inoculations are all done with a loop. The first of these three procedures is the inoculation of B7 into the nutrient broth agar. First, the tip of the loop is flamed in the blue cone of the Bunsen burner at a 45-degree angle to sterilize and prep it for inoculating the specimen. After allowing the loop to cool, the media being used is then flamed at the lip and a loopful of the bacteria is obtained. Insert the loopful of bacteria into the nutrient broth agar and be cautious of the loop hitting the sides upon entry or removal. …show more content…
Re-flame the loop to re-sterilize it for the next procedure. After allowing the loop to cool, it is then ready to inoculate the nutrient agar slant. The nutrient agar slant follows the same procedures of the nutrient broth agar, with a few exceptions. The nutrient agar slant still requires the process of flaming the loop and the lip of the tube before and after inoculation, but the direct contact between the two is different because it is gelatin based solid rather than a liquid. After the flaming of the loop, lip of the tube and obtaining a loopful of bacteria, it is then inserted into the media and makes contact by a simple streak, or line, from the bottom of the agar to the top. The media is then re-flamed and closed while the loop is re-flamed for sterilization. Finally, the inoculation of the nutrient agar plate. The plate is inoculated by the method of the 4-quadrant streak. After following the aseptic technique of flaming the loop, since the plate does not need to be flamed here, a loopful of bacteria is then obtained and ready for the streak. Starting from one side of the plate, the loop is swept back and forth in a zig zag