Sulfamethazine remediation potential of Arabidopsis thaliana root
ABSTRACT
The use of antibiotics in animals either as a growth promoting or as a disease treating agent has become a worldwide trend. Antibiotics being stable bioactive compounds are not fully absorbed into the animal system and are released into the environment through animal manure. This study aims to discern the effect of one such veterinary antibiotic, sulfamethazine, on Arabidopsis thaliana root and also the possible phytoremediation capacity of Arabidopsis thaliana. Plants are grown under controlled condition in artificial, Murashigae and Skoog, medium spiked with three varying levels of sulfamethazine concentration (1 mg/ml, 5 mg/ml and 10 mg/ml). The roots of 14 day …show more content…
2 we can see the difference between the cellular anatomies of test plants from that of control. In case of control we are able to clearly distinguish the various cellular components without any difficulty. However, in case of SA treated plants there seems to be no clear demarcation between the cellular components. During the initial exposure of plant roots to SA there is a disruption of the root’s histology by the formation of node and pocket like structure. These cellular outgrowths can very well be the cause of the secondary root structure formation. In comparison to the initial exposure, the latter images (28 days) shows the gradual loss of the unwanted nodes and pockets. This may be due to the action of plant’s defence mechanism. Plants produce various enzymes in response to stress which cancel the toxicity of foreign compounds by breaking them down, which in return neutralizes the negative effect. Another interesting observation is the decreasing level of histological anomaly with increasing SA concentration. This observation can be tested further to determine the possible hormetic effect of SA in Arabiopsis thaliana. The TEM image of both the 14 day and 28 day old control plants showed no difference from one …show more content…
However, in the light of the results obtained and on the basis of previous findings, it is safe to establish a positive phytoremediation potential of Arabidopsis against SA. The production of reactive oxygen species (ROS) by plants in response to the antibiotic stress can be a factor involved in the remediation process. The production of ROS and its involvement in degradation of organic pollutants has been documented before (Gujarathi et al., 2005). In their study, hairy roots of Helianthus annuus (sunflower) were shown to oxidize oxytetracycline (OTC) through the action of the ROS. The probability of involvement of ROS, in the present study, weighs higher in comparison to its non-involvement. Further experiments to support this probability is one more step towards establishing the phytoremediation potential of Arabidopsis thaliana against
ABSTRACT
The use of antibiotics in animals either as a growth promoting or as a disease treating agent has become a worldwide trend. Antibiotics being stable bioactive compounds are not fully absorbed into the animal system and are released into the environment through animal manure. This study aims to discern the effect of one such veterinary antibiotic, sulfamethazine, on Arabidopsis thaliana root and also the possible phytoremediation capacity of Arabidopsis thaliana. Plants are grown under controlled condition in artificial, Murashigae and Skoog, medium spiked with three varying levels of sulfamethazine concentration (1 mg/ml, 5 mg/ml and 10 mg/ml). The roots of 14 day …show more content…
2 we can see the difference between the cellular anatomies of test plants from that of control. In case of control we are able to clearly distinguish the various cellular components without any difficulty. However, in case of SA treated plants there seems to be no clear demarcation between the cellular components. During the initial exposure of plant roots to SA there is a disruption of the root’s histology by the formation of node and pocket like structure. These cellular outgrowths can very well be the cause of the secondary root structure formation. In comparison to the initial exposure, the latter images (28 days) shows the gradual loss of the unwanted nodes and pockets. This may be due to the action of plant’s defence mechanism. Plants produce various enzymes in response to stress which cancel the toxicity of foreign compounds by breaking them down, which in return neutralizes the negative effect. Another interesting observation is the decreasing level of histological anomaly with increasing SA concentration. This observation can be tested further to determine the possible hormetic effect of SA in Arabiopsis thaliana. The TEM image of both the 14 day and 28 day old control plants showed no difference from one …show more content…
However, in the light of the results obtained and on the basis of previous findings, it is safe to establish a positive phytoremediation potential of Arabidopsis against SA. The production of reactive oxygen species (ROS) by plants in response to the antibiotic stress can be a factor involved in the remediation process. The production of ROS and its involvement in degradation of organic pollutants has been documented before (Gujarathi et al., 2005). In their study, hairy roots of Helianthus annuus (sunflower) were shown to oxidize oxytetracycline (OTC) through the action of the ROS. The probability of involvement of ROS, in the present study, weighs higher in comparison to its non-involvement. Further experiments to support this probability is one more step towards establishing the phytoremediation potential of Arabidopsis thaliana against