Next the EtOH was removed leaving the pellet and allowing the pellet to dry by uncovering the test tube and placing it upside down in a kimwipe for five minutes at room temperature. Following that the pellet was resuspened in 50 ul of TE, and by tapping the pellet was dissolved. After it was place in the heat block for 1-2 minutes at 65˚C and continuously tap the pellet until it was completely dissolved to ensure that this DNA would react with the 1 ul PCR reaction. Later the nanodrop quantified the DNA amount of the sample and the yield should have been estimated to be around 3 ug from the 5
Next the EtOH was removed leaving the pellet and allowing the pellet to dry by uncovering the test tube and placing it upside down in a kimwipe for five minutes at room temperature. Following that the pellet was resuspened in 50 ul of TE, and by tapping the pellet was dissolved. After it was place in the heat block for 1-2 minutes at 65˚C and continuously tap the pellet until it was completely dissolved to ensure that this DNA would react with the 1 ul PCR reaction. Later the nanodrop quantified the DNA amount of the sample and the yield should have been estimated to be around 3 ug from the 5