How Does Austrate Concentration Affect The Rate Of Reaction

Decent Essays
Reviewing my data table and graph results it is clear to see that as the substrate concentration increases so does the rate of reaction. The catalysing of enzymes involves molecular movement of the substrate and enzymes in an aqueous solution. Therefore the substrate and enzymes are moving randomly within the solution (Brownian motion). By increasing the concentration of the substrate particles the rate of reaction is increased as the substrate is much more likely to find an active site. This results in more reactions occurring which then produces more product (oxygen) which causes the filter paper disks to rise to the surface of the solution at an accelerated rate. This increase in the reaction speed can be viewed by calculating the percentage …show more content…
However the percentage of increases from sixty to eighty is ninety percent and from eighty to one hundred percent concentration is one hundred and sixty three percent. This shows an exponential percentage increase in product production by increasing the substrate concentration. Although not viewed in the practical if further concentrations of substrate were introduced to this experiment it would be expected that the rate of reaction would level off. This levelling off point is known as the point of saturation. Which means that the amount of substrate in the solution now exceeds the number of active sites available for the substrate. This is because the maximum number of reactions are being done instantaneously so any extra substrate has to wait until an active site become available. Reviewing the reaction rate graph it can be noted that for one hundred percent concentration the error bar is indicating a large amount of variability compared to the error bars of other concentrations. These results could be accounted for with regard to physical or human error during the practical examination, for example measuring reaction time to the closest 0.01 of a second rather than 0.1, the disk sticking to the base and the thin girth of the tube hampering disk

Related Documents

  • Superior Essays

    Albumin Synthesis

    • 1183 Words
    • 5 Pages

    The average absorbance rate in figure 2 presents the different levels of reaction in terms of the chemical compounds. Comparing the average values (Team 1 representing our results) there is not much of a pattern when viewing the absorbance for each specific test tube for each team. Although, there are some values that were around the same range including test tube 2. As for enzyme change, the high amount of enzymes increases the absorbance that shows an increase in reaction as seen in test tube 4 (figure 1). However, because of the decrease at 30 minutes the results take a shift.…

    • 1183 Words
    • 5 Pages
    Superior Essays
  • Improved Essays

    Analysis Of Alka Seltzer

    • 1720 Words
    • 7 Pages

    The third slope decreased by 0.038 grams. The reason why the rate of reaction is happening at a faster rate is because of the Sodium Hydroxide. During…

    • 1720 Words
    • 7 Pages
    Improved Essays
  • Decent Essays

    The pop-tab Lab Arnav Wadhawan Ridley College Gr. 9 Science Oct,1,2015 Inquire Some of the factors that that may affect the rate that the chemical reaction is the quantity of each chemical, the temperature of the chemicals and the pressure inside the container that the chemicals are in. Design Section Evaluation section…

    • 121 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    My results do not really make sense due to the fact that they didn't go for the glucose solution maybe it wasn’t a high enough concentration to attract…

    • 1255 Words
    • 6 Pages
    Improved Essays
  • Improved Essays

    Experiment 4: Kinetics of Nucleophilic Substitutions 1. Determine the effect of varying [OH-] on the rate of the reaction. To do this, you should complete the following table: Experiment [tBuCl]0 (M) [OH-]0 (M ) Time (s) Reaction rate (M/s) Rate constant (s-1) 1 0.03 0.003 65 4.89 · 10-5 1.62 · 10-3 2 0.03 0.006 213 3.15 · 10-5 1.05 · 10-3 3 0.03 0.009 362 2.96 · 10-5 9.85 · 10-4 What is the dependence of [OH-] on the rate law? The effect of the increased or decreased [OH-] on the rate law is very minimal.…

    • 1172 Words
    • 5 Pages
    Improved Essays
  • Decent Essays

    In most cases, a chemical reaction is able to occur in more than one way; enzyme kinetics studies the effect of how biological catalysts can influence biochemical reactions. Enzymes play an important role in regulating metabolic pathways in the human body and without enzymes they will not function efficiently. The rate of a reaction is enhanced by enzyme activity which provides alternate pathways for the reaction to proceed by.…

    • 177 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    Tube one was our positive control. The enzyme was fully efficient and there was no inhibitor. It is expected that tube one will be catalyzed the quickest. Tube two was our negative control. We needed to observe what would occur in the spectrophotometer if the enzyme couldn’t catalyze the reaction and so we added a denatured enzyme to view the effects of no reaction.…

    • 718 Words
    • 3 Pages
    Improved Essays
  • Superior Essays

    Reaction Lab

    • 1527 Words
    • 7 Pages

    What effects do different conditions have on the rate of reaction? Clarissa Reyna University of Texas Rio Grande Valley October 31, 2017 T.A: Marcia Calderon ABSTRACT: The motive behind this two week lab experiment was to analyze what different conditions have on the rate of reaction.…

    • 1527 Words
    • 7 Pages
    Superior Essays
  • Improved Essays

    Liver Lab

    • 544 Words
    • 3 Pages

    The difference between the two graphs is the speed of the reaction. The temperatures of the two tests were very similar throughout the experiment. when doing this experiment one should have a paper towel ready to wipe the side of the test tube to be able to read the temperature. Conclusion: This experiment tested the difference between a untreated and treated catalase enzyme.…

    • 544 Words
    • 3 Pages
    Improved Essays
  • Great Essays

    In this part of the lab, we pour 20 mL of Hydrogen Peroxide and 1 mL of yeast catalase in the reaction chamber and then submerge it in the water bath. After it was done, it can be concluded that the gas levels increased because when we first started timing it, the gas levels were at 76, but as time went by, the gas levels started to increase and in the end of the last interval, the gas level increased all the way up to 100. This result occurred because there were many active sites that were empty for the substrate to bind to and since they binded to the active sites, it started increasing a lot at the beginning. However, when the active sites started getting more occupied, the substrate could not bind to as much active sites, so the gas levels started to increase alittle less as time went on and in the end, there was no active sites that were…

    • 1980 Words
    • 8 Pages
    Great Essays
  • Decent Essays

    Finally, create two graphs, one to display data from table 1a, and the other from 2a. Graph the number of toothpicks over the time it took. Label the axis accordingly and create a legend differentiate each set of data. Calculate the reaction rate for each time interval (divide the total number of toothpicks broken by the change in time), then record and graph.…

    • 388 Words
    • 2 Pages
    Decent Essays
  • Decent Essays

    Acetylthiocholine Essay

    • 237 Words
    • 1 Pages

    The enzyme inhibition (%) was calculated from the rate of absorbance change with time (V= Abs/Δt) the calculation as follows.…

    • 237 Words
    • 1 Pages
    Decent Essays
  • Improved Essays

    The purpose of conducting this experiment was to explore how different factors affect the reaction rate of enzymes reacting with their corresponding substrates in order to learn more about how enzymes function in different environments. The independent variables investigated in this experiment were the concentration of different substrates, the temperature of the environment, and the effect of a catalyst on the reaction rate. The dependent variable for all of the investigations was the time it took for the reaction to occur. To investigate the effect of the concentration of the substrate on the reaction time, four test tubes were used.…

    • 1027 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Lactate dehydrogenase (LDH) is an oxidoreductase found in both eukaryotes and prokaryotes. LDH catalyses the formation of lactate and NAD+ from pyruvate and NADH, during the last step of anaerobic glycolysis. The reaction is also catalysed in the other direction by LDH during the Cori cycle. This reaction can be assayed using spectrophotometric techniques; peak absorbance of NADH is at 340nm, but the peak absorbance of NAD+ is 259nm (Powers, et al., 2007). Enzyme assays are performed to measure the rate of an enzyme-catalysed reaction, known as the enzyme activity.…

    • 1627 Words
    • 7 Pages
    Improved Essays
  • Superior Essays

    Potato Reaction Lab Report

    • 1820 Words
    • 8 Pages

    Throughout each test, manganese dioxide had a higher reaction rate than the potato. In data table two, the new potato piece and new H₂O₂ had a reaction rate of 2.5 out of 3 and the manganese and new H₂O₂ had a reaction rate of 3. Additionally, the 1 mashed potato piece had a reaction rate of 2 out of 5 and 1 scoop of manganese dioxide had a reaction rate of 3. Two mashed potato pieces had a reaction rate of 4 and 2 scoops of manganese dioxide had a reaction rate of 5. Three mashed potatoes had a reaction rate of 4.5, and 3 scoops of manganese dioxide had a reaction rate of 5.…

    • 1820 Words
    • 8 Pages
    Superior Essays