For this experiment, we will try to find the effects of caffeine on the replication process of DNA. In order to identity the effect of caffeine on DNA synthesis an experiment must be formed. In this experiment, cells must be soaked into a certain concentration of caffeine for some time. However, before this a tracker must be placed on the DNA within the cells. This way the synthesized DNA strands can be identified. After the DNA is labeled and incubated in caffeine, the DNA strands need to be split. We will use a lysin to deconstruct the DNA and then place it on a gradient. After this we will place the gradient in a motor and rotate the DNA. This way the DNA will be fractionated and then the molecular weight can be calculated.
Another possible experiment could be to take L cells and soak …show more content…
The supplies from the second proposed experiment is not as expensive as the one from the first proposed experiment.
The first proposed experiment can be explained in more detail. We will test on mouse lymphoma cells so we can predict risk assessment on vivo testing. Vivo testing refers to experimentation using a whole living organism (M Lloyd, 2012). We will use 1,3,7-Trimethylpurine -2,6-dione (caffeine) at a concentration of .3 mg/ml or 1.6 mM. This is because as stated above, caffeine at the concentration of 1.6 mM affects irradiated cells. We will use pulse labeling technique for this experiment. Pulse labeling is a biochemistry technique which aids in the process of finding the presence of a target molecule by labeling the sample with a radioactive compound. By using the labeling technique, we will be able to monitor the effect of caffeine when the process of DNA synthesis occurs. We will use thymidine as a radioactive label for the DNA antecedent. This will form a different gradient for this experiment. The gradient that will form would be the alkaline sucrose gradient. Alkaline sucrose gradient sedimentation is a technique for dividing macromolecules like DNA and
Another possible experiment could be to take L cells and soak …show more content…
The supplies from the second proposed experiment is not as expensive as the one from the first proposed experiment.
The first proposed experiment can be explained in more detail. We will test on mouse lymphoma cells so we can predict risk assessment on vivo testing. Vivo testing refers to experimentation using a whole living organism (M Lloyd, 2012). We will use 1,3,7-Trimethylpurine -2,6-dione (caffeine) at a concentration of .3 mg/ml or 1.6 mM. This is because as stated above, caffeine at the concentration of 1.6 mM affects irradiated cells. We will use pulse labeling technique for this experiment. Pulse labeling is a biochemistry technique which aids in the process of finding the presence of a target molecule by labeling the sample with a radioactive compound. By using the labeling technique, we will be able to monitor the effect of caffeine when the process of DNA synthesis occurs. We will use thymidine as a radioactive label for the DNA antecedent. This will form a different gradient for this experiment. The gradient that will form would be the alkaline sucrose gradient. Alkaline sucrose gradient sedimentation is a technique for dividing macromolecules like DNA and